In vitro Artemisia L. plants as a source of biologically active compounds / Drobot K.O., Matvieieva N.A. 2015
The method for micropropagation of Artemisia vulgaris L., A. dracunculus L., A. annuа L. and A. tilesii Ledeb. plant was established. Cultivation of the plants on MS medium supplemented with 0.5 mg/l BAP + 0.05 mg/l NAA as well as 1 mg/l BAP + 0.1 mg/l NAA have led to 8 lateral shoots formation during 14 days. The polyfructan content and antioxidant activity (AOA) of extracts of in vitro cultivated A. vulgaris, A. dracunculus, A. annuа and A. tilesii plants were determined. A. tilesii root extracts possessed the highest fructan content, up to 16.9 mg/g fresh weight and the highest antioxidant activity level (46% compared to AOA of 0.1% ascorbic acid solution). The AOA and fructan content of in vitro cultured A. vulgaris, A. dracunculus, A. annua and A. tilesii leaves were found to be 23%, 31%, 24%, 31.7% (АОА) and 7.6 mg/l, 4.4 mg/l, 7.3 mg/l, 8.4 mg/l fresh weight (fructan content) respectively. So, in vitro cultivated Artemisia plants accumulated fructans and substances with antioxidant activity and can be used as a source of biologically active compounds.
Keywords: Artemisia spp., in vitro propagation, fructan content, DPPH-scavenging activity