https://icbge.org.ua/en/api.php?action=feedcontributions&feedformat=atom&user=SheludkoICBGE - User contributions [en]2026-04-19T00:04:46ZUser contributionsMediaWiki 1.19.24https://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2019-02-17T20:14:13Z<p>Sheludko: </p>
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<div>PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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E-mail: ysheludko@ukr.net<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – 2018; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 16 (Google Scholar, i10=18); 12 (Scopus, self-citations are excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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42. Sheludko Y.V., Gerasymenko I.M., Warzecha H. (2018) Transient expression of human cytochrome P450s 2D6 and 3A4 in Nicotiana benthamiana provides a possibility for rapid substrate testing and production of novel compounds. Biotechnol. J. (Accepted Author Manuscript) [https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700696 doi:10.1002/biot.201700696].<br />
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43. <br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2018-04-11T17:32:59Z<p>Sheludko: </p>
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<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 16 (Google Scholar, i10=18); 12 (Scopus, self-citations are excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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42. Sheludko Y.V., Gerasymenko I.M., Warzecha H. (2018) Transient expression of human cytochrome P450s 2D6 and 3A4 in Nicotiana benthamiana provides a possibility for rapid substrate testing and production of novel compounds. Biotechnol. J. (Accepted Author Manuscript) [https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700696 doi:10.1002/biot.201700696].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2018-04-11T17:31:43Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
<br />
<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
<br />
<B>Awards:</B> <br />
<br />
Stipendium of National Academy of Science of Ukraine (2003)<br />
<br />
Stipendium of President of Ukraine (2004)<br />
<br />
Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
<br />
<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
<br />
<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 16 (Google Scholar, i10=18); 12 (Scopus, self-citations are excluded)<br />
<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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42. Sheludko Y.V., Gerasymenko I.M., Warzecha H. (2018) Transient expression of human cytochrome P450s 2D6 and 3A4 in Nicotiana benthamiana provides a possibility for rapid substrate testing and production of novel compounds. Biotechnol. J. (Accepted Author Manuscript) [https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700696 doi:10.1002/biot.201700696].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2018-02-04T20:13:43Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 16 (Google Scholar, i10=18); 12 (Scopus, self-citations are excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2018-02-04T20:08:25Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 16 (Google Scholar, i10=18); 11 (Scopus, self-citations are excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-10T15:29:23Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citations are excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-09T16:49:08Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/authid/detail.uri?authorId=6603268494 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-09T16:32:08Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/cto2/main.uri?origin=savedlist&stateKey=CTOF_812971932 Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-09T15:54:12Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/cto2/main.uri?ctoId=CTODS_812971349&authors=6603268494&origin=AuthorNamesList Scopus]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-09T15:53:42Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/cto2/main.uri?ctoId=CTODS_812971349&authors=6603268494&origin=AuthorNamesList Scopus]<br />
==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-09T15:53:14Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==[https://www.scopus.com/cto2/main.uri?ctoId=CTODS_812971349&authors=6603268494&origin=AuthorNamesList Scopus]<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-08T14:02:27Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 134 scientific publications including 39 publications in international peer reviewed scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
<br />
40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
<br />
41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T21:03:18Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 115 scientific publications including 32 publications in international peer review scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 12 (Google Scholar, i10=12); 9 (Scopus, self-citation excluded)<br />
<br />
==[https://scholar.google.com.ua/citations?hl=en&user=kK8edmAAAAAJ&imq=Iryna+Gerasymenko&scilu=15214582795085069679:0&scisig=AMstHGQAAAAAWVgMaCCx_6k2W6EpUQG-616YIOfeCP4W&gmla=AJsN-F5QUMFJvI6NUpK3YIN-5155y_hcTIn-4i1U5TLCrW4K9CIFQdcY9n6X2LcBR-u140t_GiEcrM35NiFKErZyQ9OmYjoD-_qMj-uUUI1uNcNNkSSfU9M&sciund=1770499563793843521&cstart=-60&pagesize=80 Google Scholar Citation]==<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T21:02:35Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 115 scientific publications including 32 publications in international peer review scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 12 (Google Scholar, i10=12); 9 (Scopus, self-citation excluded)<br />
<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T21:00:55Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 115 scientific publications including 32 publications in international peer review scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 12 (Google Scholar, i10=12); 9 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-07-01T15:51:36Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
<br />
<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
<br />
<B>Awards:</B> <br />
<br />
Stipendium of National Academy of Science of Ukraine (2003)<br />
<br />
Stipendium of President of Ukraine (2004)<br />
<br />
Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
<br />
<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
<br />
<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 134 scientific publications including 39 publications in international peer review scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
<br />
==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
<br />
2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
<br />
3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
<br />
4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
<br />
7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
<br />
8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
<br />
9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
<br />
10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
<br />
11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
<br />
12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
<br />
13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
<br />
14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
<br />
15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
<br />
16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
<br />
19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
<br />
20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
<br />
40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
<br />
41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:51:31Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 115 scientific publications including 32 publications in international peer review scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 15 (Google Scholar, i10=17); 10 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:34:54Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:32:17Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> December 14, 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)].<BR><br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<BR><br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<BR><br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.<BR><br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<BR><br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:31:19Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> December 14, 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)]<br />
<br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<br />
<br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
<br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
<br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:30:33Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> December 14, 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)]<br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:29:28Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> December 14, 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] <br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Iryna_M._GerasymenkoIryna M. Gerasymenko2017-07-01T15:28:39Z<p>Sheludko: </p>
<hr />
<div>PhD<br />
<br />
Scientific Associate of [[Department of genetic engineering]].<br />
<br />
<B>Iryna Mykhajlivna GERASYMENKO</B><br />
<br />
CURRICULUM VITAE<br />
<br />
Institute of Cell Biology & Genetic Engineering<br />
<br />
Natl. Academy of Sci. of Ukraine<br />
<br />
Zabolotnogo str., 148, Kiev, 03143, Ukraine;<br />
<br />
Tel/fax: +380 (44) 526 7104; 522 1787<br />
<br />
E-mail: i-gerasimenko@ukr.net<br />
<br />
http://icbge.org.ua/eng/Iryna_M._Gerasymenko<br />
<br />
<B>Year and Place of Birth:</B> December 14, 1973, Kiev, Ukraine<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points) <br />
<br />
<B>Education, Scientific Degrees and Titles:</B><BR><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;<BR><br />
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).<br />
<br />
<B>Professional Experience:</B><BR><br />
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR><br />
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);<BR> <br />
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany). <br />
<br />
<B>Grant support:</B><BR> <br />
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”<BR><br />
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)<BR><br />
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)<BR><br />
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”<BR><br />
<br />
<B>Teaching Experience:</B><br />
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”<br />
<br />
Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user. <br />
<br />
<B>Awards:</B> <br />
• Stipendium of National Academy of Science of Ukraine (2002)<BR><br />
• Stipendium of President of Ukraine (2008)<br />
<br />
<B>Fields of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology<br />
<br />
<B>Scientific Method Experience:</B> complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.<br />
<br />
<B>Publications:</B> 28 publications in peer-reviewed scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 7 (Scopus, self-citation excluded)<br />
<br />
<B>Selected Scientific Publications:</B><BR><br />
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.<BR><br />
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.<BR><br />
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<BR><br />
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<BR><br />
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<BR><br />
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<BR><br />
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<BR><br />
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.<BR><br />
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.<BR><br />
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.<BR><br />
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.<BR><br />
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.<BR><br />
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] <br />
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.<br />
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
<br />
<br />
[[Category:Staff|Gerasymenko]]<br />
[[Category:Staff of the Department of genetic engineering|Gerasymenko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Gerasymenko]]<br />
<br />
[[uk:Герасименко Ірина Михайлівна]]<br />
[[ru:Герасименко Ирина Михайловна]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-06-29T16:01:59Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 15 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-06-20T19:24:54Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 14 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. [https://link.springer.com/article/10.1007/s10529-017-2336-z DOI:10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-04-24T15:38:32Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 14 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
<br />
8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotechnology Letters: 1-9. [http://link.springer.com/article/10.1007/s10529-017-2336-z DOI: 10.1007/s10529-017-2336-z].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-04-24T15:36:55Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 14 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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41. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotechnology Letters: 1-9. 10.1007/s10529-017-2336-z<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-03-02T21:02:14Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 14 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
<br />
20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2017-02-22T08:10:13Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 14 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
<br />
22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
<br />
23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
<br />
25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
<br />
26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7.<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-09-06T16:15:49Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 13 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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==<B>List of major publications</B><BR>==<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2016) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2016) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. (in press).<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-09-06T16:14:43Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 13 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2016) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. [http://link.springer.com/article/10.1007/s11248-016-9980-2 DOI: 10.1007/s11248-016-9980-2].<br />
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40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2016) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. (in press).<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-09-06T15:38:46Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 13 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2016) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. DOI: 10.1007/s11248-016-9980-2.<br />
<br />
40. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2016) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. (in press).<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-09-06T15:38:19Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 39 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 13 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
39. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2016) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. DOI: 10.1007/s11248-016-9980-2.<br />
<br />
40. 131. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2016) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. (in press).<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yurij_V._SheludkoYurij V. Sheludko2016-09-06T08:20:39Z<p>Sheludko: Replacing page with '[http://icbge.org.ua/eng/Yuriy_V._Sheludko]'</p>
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<div>[http://icbge.org.ua/eng/Yuriy_V._Sheludko]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2016-09-06T08:16:47Z<p>Sheludko: Removing all content from page</p>
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<div></div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-07-06T14:37:19Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 38 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 13 (Google Scholar); 10 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2016-06-08T15:21:14Z<p>Sheludko: </p>
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==Laboratory was established in 2009 году==<br />
Group performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
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==Structure:==<br />
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Senior scientific associate PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
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==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
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[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
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In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
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Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
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[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
<br />
Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship for experienced researchers HERMES</B> (2015)<br />
<br />
</div><br />
<br />
<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
<br />
*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
<br />
*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
<br />
*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
<br />
*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
<br />
*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
<br />
*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
<br />
[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2016-04-25T18:14:44Z<p>Sheludko: </p>
<hr />
<div><div style='float:left;width:450px;height:1200px;'><br />
==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
<br />
==Structure:==<br />
<br />
Senior scientific associate PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
<br />
==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
</div><br />
<br />
<div style='float:right;width:450px;height:1200px;'><br />
<br />
[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
<br />
<BR><br />
In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
<BR> <br />
Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
<br />
<br />
[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
<br />
Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship for experienced researchers HERMES</B> (2015)<br />
<br />
</div><br />
<br />
<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
<br />
*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
<br />
*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
<br />
*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
<br />
*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
<br />
*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
<br />
*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
<br />
[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-04-09T18:28:57Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
<br />
<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
<br />
<B>Awards:</B> <br />
<br />
Stipendium of National Academy of Science of Ukraine (2003)<br />
<br />
Stipendium of President of Ukraine (2004)<br />
<br />
Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
<br />
<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
<br />
<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 38 publications in international scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 13 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
<br />
==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
<br />
2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
<br />
3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
<br />
4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
<br />
7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
<br />
8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
<br />
11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
<br />
12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
<br />
14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
<br />
15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
<br />
16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
<br />
19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
<br />
20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
<br />
21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
<br />
23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
<br />
25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
<br />
26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-04-09T18:27:51Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology and Metabolic Engineering, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
<br />
<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
<br />
<B>Awards:</B> <br />
<br />
Stipendium of National Academy of Science of Ukraine (2003)<br />
<br />
Stipendium of President of Ukraine (2004)<br />
<br />
Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
<br />
<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
<br />
<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 13 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
<br />
==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
<br />
2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
<br />
3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
<br />
4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
<br />
7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
<br />
8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
<br />
9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
<br />
10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
<br />
11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
<br />
12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
<br />
13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
<br />
14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
<br />
15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
<br />
16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
<br />
19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
<br />
25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
<br />
26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-02-03T12:37:23Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
<br />
• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
<br />
• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
<br />
<B>Professional Experience:</B><br />
<br />
• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
<br />
• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
<br />
• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
<br />
• Technische Universität Darmstadt, Plant Biotechnology, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
<br />
<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
<br />
<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
<br />
<B>Awards:</B> <br />
<br />
Stipendium of National Academy of Science of Ukraine (2003)<br />
<br />
Stipendium of President of Ukraine (2004)<br />
<br />
Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
<br />
<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
<br />
<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
<br />
<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
<br />
<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
<br />
<B>H-index:</B> 13 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
<br />
==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
<br />
1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
<br />
3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
<br />
4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
<br />
7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
<br />
8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
<br />
9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
<br />
10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
<br />
11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
<br />
12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
<br />
13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
<br />
14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
<br />
15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
<br />
16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
<br />
19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
<br />
20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
<br />
21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
<br />
22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
<br />
23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
<br />
25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2016-02-03T12:34:01Z<p>Sheludko: </p>
<hr />
<div>Senior scientific associate of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – 01.2016;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 12 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
<br />
19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
<br />
20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
<br />
21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
<br />
22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
<br />
23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
<br />
24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
<br />
25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
<br />
26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2016-02-03T12:32:14Z<p>Sheludko: </p>
<hr />
<div><div style='float:left;width:450px;height:1200px;'><br />
==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
<br />
==Structure:==<br />
<br />
Senior scientific associate PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
<br />
==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
</div><br />
<br />
<div style='float:right;width:450px;height:1200px;'><br />
<br />
[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
<br />
<BR><br />
In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
<BR> <br />
Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
<br />
<br />
[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
<br />
Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship for experienced researchers HERMES</B> (2015)<br />
<br />
</div><br />
<br />
<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
<br />
*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
<br />
*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
<br />
*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
<br />
*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
<br />
*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
<br />
*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
<br />
[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2015-12-09T15:26:36Z<p>Sheludko: </p>
<hr />
<div><div style='float:left;width:450px;height:1200px;'><br />
==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
<br />
==Structure:==<br />
Head of laboratory, PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
Diploma student, 1st rank engineer(1/2 rate) [[Iryna A. Arbuzova]]<br />
<br />
==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
</div><br />
<br />
<div style='float:right;width:450px;height:1200px;'><br />
<br />
[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
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<BR><br />
In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
<BR> <br />
Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
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[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
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Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship for experienced researchers HERMES</B> (2015)<br />
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<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
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*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
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*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
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*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
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*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
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*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
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*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
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[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2015-06-22T13:40:18Z<p>Sheludko: </p>
<hr />
<div>Head of [[Laboratory of Natural Product Biosynthesis Systems]] of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – up to now;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062); 2015-2019 "Studying on plant stress adaptation mechanisms and generation of resistant lines using experimental transgenesis protocols (№ UISTEI 0115U004171); .<br />
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<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 12 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
<br />
26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
<br />
27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2015-06-22T13:36:57Z<p>Sheludko: </p>
<hr />
<div><div style='float:left;width:450px;height:1200px;'><br />
==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
<br />
==Structure:==<br />
Head of laboratory, PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
Scientific associate, PhD, [[Yana R. Sindarovska]]<br><br />
Principal engineer [[Mariya G. Mazur]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
Diploma student, 1st rank engineer(1/2 rate) [[Iryna A. Arbuzova]]<br />
<br />
==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
</div><br />
<br />
<div style='float:right;width:450px;height:1200px;'><br />
<br />
[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
<br />
<BR><br />
In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
<BR> <br />
Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
<br />
<br />
[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
<br />
Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship for experienced researchers HERMES</B> (2015)<br />
<br />
</div><br />
<br />
<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
<br />
*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
<br />
*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
<br />
*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
<br />
*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
<br />
*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
<br />
*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
<br />
[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2015-06-22T13:36:22Z<p>Sheludko: </p>
<hr />
<div><div style='float:left;width:450px;height:1200px;'><br />
==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
<br />
==Structure:==<br />
Head of laboratory, PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
Scientific associate, PhD, [[Yana R. Sindarovska]]<br><br />
Principal engineer [[Mariya G. Mazur]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
Diploma student, 1st rank engineer(1/2 rate) [[Iryna A. Arbuzova]]<br />
<br />
==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
</div><br />
<br />
<div style='float:right;width:450px;height:1200px;'><br />
<br />
[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
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<BR><br />
In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
<BR> <br />
Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
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[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
<br />
Dr. [[Yuriy V. Sheludko]] was awarded <B>Georg Forster Research Fellowship (HERMES) for experienced researchers</B> (2015)<br />
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<div style='float:center;width:900px;'><br />
<br />
==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
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*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
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*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
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*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
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*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
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*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
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*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
</div><br />
<br />
[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
<br />
[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2015-06-22T11:49:33Z<p>Sheludko: </p>
<hr />
<div>Head of [[Laboratory of Natural Product Biosynthesis Systems]] of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
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Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
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<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – up to now;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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• Technische Universität Darmstadt, Plant Biotechnology, Schnittspahnstrasse 4 Darmstadt, 64287, Germany, research work on the project "Engineering of plant alkaloid metabolism with heterologous cytochrome P450 enzymes" according to Georg Forster Research FellowshipP rogramme HERMES supported by Alexander von Humboldt Foundation, 06.2015 - up to now.<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062).<br />
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<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 12 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2015-06-21T16:00:30Z<p>Sheludko: </p>
<hr />
<div>Head of [[Laboratory of Natural Product Biosynthesis Systems]] of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
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<B>Education, Scientific Degrees and Titles:</B><br />
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• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – up to now;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062).<br />
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<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 12 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
<br />
29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
<br />
37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Yuriy_V._SheludkoYuriy V. Sheludko2015-06-21T15:57:00Z<p>Sheludko: </p>
<hr />
<div>Head of [[Laboratory of Natural Product Biosynthesis Systems]] of [[Department of genetic engineering]]<BR><br />
PhD (2004)<BR><br />
Senior Research Assistant (2010)<BR><br />
<br />
Phone: +380 44 522-1787<BR><br />
E-mail: ysheludko@ukr.net<br />
<br />
<B>Year of Birth</B>: 1972<br />
<br />
<B>Education, Scientific Degrees and Titles:</B><br />
<br />
• Taras Shevchenko Kiev State University, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1994;<br />
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• Ph.D. in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2004 <br />
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• Senior Research Assistant in Biotechnology, Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine, 2010<br />
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<B>Professional Experience:</B><br />
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• Institute of Cell Biology and Genetic Engineering, 1994 – up to now; Head of Laboratory of Natural Product Biosynthesis Systems of Department of Genetic Engineering, 2009 – up to now;<br />
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• Institute of Pharmacy (Mainz, Germany), participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269), 1997 –1999;<br />
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• Institute of Pharmacy (Mainz, Germany), research work, 1999 – 2002; 2006;<br />
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• Taras Shevchenko National University of Kiev, ESC Institute of Biology, invited lecturer for “Plant Biochemistry”, “Plant Secondary Metabolism”, “Genetic Basis of Biotechnology”, 2007 – 2013<br />
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<B>Field of scientific interests:</B> Plant Cell Culture / Plant biotechnology / Genetic transformation / Transient expression / Medicinal plants / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Alkaloids, Indole alkaloids / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems / Stress resistance in plants / Gene expression regulation elements / High performance liquid chromatography of secondary metabolites<br />
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<B>Scientific Method Experience:</B> transient expression and genetic transformation, in vitro plant cell culture, protein extraction and purification, enzymatic activity analysis, HPLC, FPLC, secondary metabolite structure elucidation with spectroscopic methods (MS, NMR), GE of proteins nd nucleic acids, basic protocols of PCR and molecular cloning<br />
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<B>Awards:</B> <br />
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Stipendium of National Academy of Science of Ukraine (2003)<br />
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Stipendium of President of Ukraine (2004)<br />
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<B>Languages:</B> Russian, Ukrainian - native, English – substantial<br />
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<B>Computer Experience:</B> Windows, MS Office, Adobe Photoshop, Corel Draw, Internet – advanced user, basic experience in web design<br />
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<B>Grant support:</B> NASU-RFBR 2008-2009 № 08-04-90410_ukr “Studying on the mechanisms of the higher plant abiotic stress resistance due to heterologous expression of cyanobacteria genes” (№ UISTEI 0108U003265); <br />
GFBR of Ukraine - RFBR 2011-2012 “Molecular design of system for heterlogous expression of proteins using thermostable lichenase of Clostridium thermocellum as a reporter and carrier” (№ Ф40.4/021, № UISTEI 0111U004979); <br />
grant of complex interdisciplinary program «Fundamental base of molecular and cell biotechnologies» 2010-2014 “Studying on the physiological function of different substrate specificity desaturase genes in plants under normal and abiotic stress conditions” (№ UISTEI 0110U006062).<br />
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<B>Publications:</B> 36 publications in international scientific journals, 2 patents of Ukraine;<br />
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<B>H-index:</B> 12 (Google Scholar); 8 (Scopus, self-citation excluded)<br />
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==<B>List of major publications</B><BR>==<br />
==[http://scholar.google.com.ua/citations?hl=ru&user=K-R1C3oAAAAJ&view_op=list_works&pagesize=100 Google Scholar Citation]==<br />
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1. Шелудько Ю.В., Костенюк И.А. (1994) Динамика роста и содержание суммы алкалоидов в трансгенной корневой культуре раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика , 28, 35-38. [[Media:Sheludko_1994.pdf|Download/скачать полную версию, pdf]]<br />
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2. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1996) Сpавнительный экспеpиментальный анализ пpостого метода количественного опpеделения суммы индольных лк лоидов. Химико-фармацевтический журнал, 4, 38-42.<br />
[[Media:Sheludko_1996.pdf|Download/скачать полную версию, pdf]]<br />
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3. Шелудько Ю.В., Герасименко И.М., Костенюк И.А. (1998) Исследование влияния салициловой кислоты на синтез индольных лкалоидов трансгенной корневой культурой раувольфии змеиной (Rauwolfia serpentina Benth.). Цитология и генетика, 32, 43-50.<br />
[[Media:Sheludko_1998.pdf|Download/скачать полную версию, pdf]]<br />
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4. [http://www.springerlink.com/content/jedv4yktaeyaund9/ Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918. DOI: 10.1007/s002990050683]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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5. [https://www.thieme-connect.com/DOI/DOI?10.1055/s-2000-8618 Sheludko Y., Gerasimenko I., Platonova O. (2000) Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina x Rhazya stricta. Planta Med., 66, 656-659]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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6. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.<br />
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7. Gerasimenko I., Sheludko Y., Unger M., and Stöckigt J. (2001) Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications. Phytochem. Anal., 12, 96-103.<br />
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8. Hinse C., Sheludko Y., Provenzani A., Stöckigt J. (2001) In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling. J. Am. Chem. Soc., 123, 5118-5119.<br />
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9. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: the first naturally occurring alkaloid of the raumacline group. Planta Med., 68, 435-439.<br />
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10. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.<br />
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11. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.<br />
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12. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.<br />
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13. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.<br />
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14. Sindarovska Y. R., Gerasymenko I. M., Sheludko Y. V., Bannikova M. A., Komarnytskyy I. K., Kuchuk N. V. (2005) Transgenic plants regenerated from hairy roots of Nicotiana benthamiana: a promising host for transient expression of foreign proteins. Cytology and Genetics, 39 (N6), 9-14.<br />
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15. Шелудько Ю. В., Сіндаровська Я. Р., Герасименко І. М., Банникова М. О., Олевинська З.М., Співак М. Я., Кучук М. В. (2006) Agrobacterium-опосередкована транзієнтна експресія: перспективний підхід для масштабної продукції рекомбінантних білків у рослинах. Наука та інновації, 2 (N6), 65-76.<br />
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16. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21075/abstract;jsessionid=05221409B44A36C3739227B34B66C1D1.d03t04 Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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17. [http://www.springerlink.com/content/c247m58g1144025n/ Sindarovska Y. R., Sheludko Y. V., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2008) Purifikation of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior. Cytol. Genet., 42 (N2), 16-20]. Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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18. Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stöckigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol., 2008, 67, 455-467.<br />
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19. Sheludko Y.V. (2008) Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants. Recent Patents on Biotechnology. 2, 198-208. [http://www.benthamscience.com/biot/samples/BIOT%202-3/BIOT-2-3-Sheludko.pdf?BIOT.. скачать полную версию, pdf].<br />
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20. Гориславец С.М., Рисованная В.И., Шелудько Ю.В. () Анализ окрашенных технических сортов винограда по антоциановым профилям экстрактов ягод и каллусных культур. "Экосистемы Крыма, их охрана и оптимизация", 2008, 18, 66-74.<br />
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21. Пат. 31239 Україна, МПК A 01 H 5/00, A 01 H 5/12, C 07 K 14/00, C 12 N 15/63, C 12 N 15/84, C 12 P 21/00. Спосіб одержання рекомбінантного білка шляхом транзієнтної експресії трансгену в рослині виду Nicotiana excelsior / Сіндаровська Я.Р., Шелудько Ю.В., Герасименко І.М., Кучук М.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ. – № u 2007 14867; заявл. 27.12.07; опубл. 25.03.08, Бюл. № 6.<br />
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22. Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk. Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 2010, 101, 99-103.<br />
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23. Sheludko Y. V. Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Цитология и генетика, 2010, 44 (№ 1), 52-60.<br />
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24. Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.<br />
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25. Пат. 51842 Україна, МПК C 12 N 15/00, C 12 Q 1/25, C 12 Q 1/68. Спосiб детекції рекомбiнантного гена ацил-лiпlдної десатурази, злитої з термостабiльною лiхеназою, в генетично модифiкованiй рослинi методом мультиплексної полiмеразної ланцюгової реакції / Герасименко І.М., Сіндаровська Я.Р., Шелудько Ю.В., Бердічєвєць І. Н., Шімшілашвілі Х. Р., Голдєнкова-Павлова І.В.; заявник та патентовласник Інститут клітинної біології та генетичної інженерії НАНУ та Інстітут общєй гєнєтікі ім. Н. І. Вавілова РАН. –заявл. 23.11.09, заявка № u 2009 12102; заявл. 25.11.2009; опубл. 10.08.2010, Бюл. № 15.<br />
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26. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293.<br />
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27. [http://www.springerlink.com/content/y310066m08w1043j/ Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol. Genet. 44 (N5), 313-316.]<br />
Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
28. Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion. Plant Cell, Tissue and Organ Culture 105, 21-27. <br />
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29. Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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30. Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
31. Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
32. Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
33. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
34. Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
35. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
36. Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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37. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
<br />
38. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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[[Category:Staff|Sheludko]]<br />
[[Category:Staff of the Department of genetic engineering|Sheludko]]<br />
[[Category:Staff of the Laboratory of Natural Product Biosynthesis Systems|Sheludko]]<br />
<br />
[[uk:Шелудько Юрій Всеволодович]]<br />
[[ru:Шелудько Юрий Всеволодович]]</div>Sheludkohttps://icbge.org.ua/eng/Laboratory_of_Natural_Product_Biosynthesis_SystemsLaboratory of Natural Product Biosynthesis Systems2015-06-21T15:56:26Z<p>Sheludko: </p>
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==Laboratory was established in 2009 году==<br />
==[[Media:2012_lab_presentation_english_2.pdf|Laboratory Presentation]]==<br />
Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. <br />
Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.<br />
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==Structure:==<br />
Head of laboratory, PhD,[[Yuriy V. Sheludko]]<br><br />
Scientific associate, PhD, [[Iryna M. Gerasymenko]]<br><br />
Scientific associate, PhD, [[Yana R. Sindarovska]]<br><br />
Principal engineer [[Mariya G. Mazur]]<br><br />
PhD student, 1st rank engineer(1/2 rate) [[Tatiana M. Kyrpa]]<br><br />
Diploma student, 1st rank engineer(1/2 rate) [[Iryna A. Arbuzova]]<br />
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==News:==<br />
[[Image:2014_Lviv.jpg|thumb|right|83x116px|]]<br />
Oral presentation of [[Anastasia Klebanovych|Klebanovych A.A.]] "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors [[Volodymyr Rudas| Rudas V.A.]], [[Iryna M. Gerasymenko|Gerasymenko I.M.]], [[Yuriy V. Sheludko|Sheludko Y.V.]], [[Maksym Vasylenko|Vasylenko M.Y.]], [[Mykola Kuchuk|Kuchuk N.V.]] from [[Laboratory of Natural Product Biosynthesis Systems]] and [[Department of genetic engineering]] as well as Klein T.M. from DuPont Pioneer AgBiotech <B>was awarded by 1 Place Diploma</B> during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS [http://bioweb.lnu.edu.ua/postup/index_en.htm <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014)], section [http://bioweb.lnu.edu.ua/postup/pdf/prizeri_2014.pdf GENETICS AND BIOTECHNOLOGY]. [http://bioweb.lnu.edu.ua/postup/pdf/pimb-tezi-2014.pdf Abstracts of congress].<br />
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[[Image:2012 springer Shel.jpg|100px|right]]<br />
<B>In 2013 Springer released book </B> [http://link.springer.com/book/10.1007/978-3-642-29974-2 Biotechnology for Medicinal Plants: Micropropagation and Improvement], including review article [http://www.springerlink.com/content/n12140177p360436/ Biosynthetic Potential of Hairy Roots for Production of New Natural Products] prepared by members of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yuriy V. Sheludko]] and Dr. [[Iryna M. Gerasymenko]]. <br />
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In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.<br />
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Request reprint: [mailto:ysheludko@ukr.net ysheludko@ukr.net]. <br />
==Honours and Awards:==<br />
[[Image:DSC_0642.JPG|thumb|center|323x216px| Award of diploma of Annual President Prize for Young Scientists 2011]] <B>20.04.2012</B> Member of the [[Laboratory of Natural Product Biosynthesis Systems]] Dr. [[Yana R. Sindarovska]] and member of [[Department of genetic engineering]] [[Maksym Vasylenko|Vasylenko M.Y.]] together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko were awarded with diploma of Annual President Prize for Young Scientists 2011 to for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. [[Pictures of the ceremony are here]].<br />
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[[Iryna M. Gerasymenko]] was awarded <B>DuPont Young Professor grant 2012</B> for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".<br />
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Dr. [[Yuriy V. Sheludko]] was awarded Georg Forster Research Fellowship (HERMES) for experienced researchers (2015)<br />
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==Recent_Publications:==<br />
*Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 [http://link.springer.com/article/10.3103/S0095452715030068 DOI: 10.3103/S0095452715030068)] Request: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sakhno L.O., Slyvets M.S., Korol N.A., Karbovska N.V., Ostapchuk A.M., SheludkoY.V., Kuchuk M.V. (2014) Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress. J. Stress Physiol. Biochem., 10(2), 24-34.[http://www.jspb.ru/issues/2014/N2/JSPB_2014_2_24-34.html Full text download]<br />
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*Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сливец М.С., Сахно Л.А., Шелудько Ю.В. Экспрессия трансгена desC цианобактерии в растениях рапса не изменяет их холодоусто йчивости (2013) Вісн. Укр. тов-ва генетиків і селекціонері 11(2), 267-275.Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. [http://www.springerlink.com/content/n12140177p360436/ DOI: 10.1007/978-3-642-29974-2_10]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.[http://www.biopolymers.org.ua/pdf/en/28/5/368/ fulltext] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. [http://www.biopolymers.org.ua/content/en/28/6/449/ abstract] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. [http://www.maikonline.com/maik/showArticle.do?auid=VAH5XT6GZ8 abstract]. Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.[http://www.springerlink.com/content/6687432j58r53117/?MUD=MP DOI: 10.3103/S009545271204007X ] Request/Запросить копию у автора: [mailto:ysheludko@ukr.net ysheludko@ukr.net].<br />
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*Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports [http://www.springerlink.com/content/8742jt5305615428/ DOI: 10.1007/s00299-010-0942-5]<br />
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*Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 [http://dx.doi.org/10.1007/s11240-010-9833-2 doi.org/10.1007/s11240-010-9833-2]<br />
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*Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. [http://www.springerlink.com/content/y310066m08w1043j/fulltext.pdf Cytol Genet] 44 (N5), 313-316<br />
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*Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293[http://www.springerlink.com/content/g378kx5868277615/?p=e6702546c1b54e42923d439ef7b5139e&pi=0 DOI 10.1007/s00216-010-3770-0].<br />
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*Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. [http://www.springerlink.com/content/m8450278k0421136/?p=02dbb8bb60fe42afadabacb62689e707&pi=10 Plant Cell, Tissue and Organ Culture (PCTOC)] 101, 99-103.<br />
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*Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. [http://www.springerlink.com/content/f144gv35004u7209/?p=40b4727fbced4116888147d82f120a9e&pi=9 Cytol Genet] 44 (N1), 52-60.<br />
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*Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. [http://www.bionet.nsc.ru/vogis/pict_pdf/2010/14-1/15.pdf Информационный вестник ВОГиС] 14 (1), 127-133.<br />
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[[Category:Department of genetic engineering]]<br />
[[Category:Laboratory of Natural Product Biosynthesis Systems]]<br />
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[[uk:Лабораторія систем біосинтезу природних сполук]]<br />
[[ru:Лаборатория систем биосинтеза природных соединений]]</div>Sheludko