In vitro Antarctic and Subantarctic Mosses - Revision history

WikiSysop at 08:20, 9 March 2015

2015-03-09T08:20:48Z

2015-03-08T12:43:32Z

2015-03-08T12:42:00Z

2013-04-04T06:09:32Z

2013-04-03T20:33:22Z

2013-04-03T20:31:33Z

2013-04-03T20:31:03Z

2013-04-03T20:28:37Z

2013-04-03T20:27:30Z

2013-04-03T20:24:50Z

← Older revision		Revision as of 08:20, 9 March 2015
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−	~~'''~~[[Nadiia Matvieieva]]''' [[Laboratory of Adaptative Biotechnology]]	+
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		+	<center><font size="+1">[[Nadiia Matvieieva]]<br>
		+	''[[Laboratory of Adaptative Biotechnology]]''
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	Interest in the study of Antarctic plants is associated with extreme conditions of this region and the influence of complex abiotic stress factors on their growing. Plants growing in vitro can serve as a model on which one can study the effect of stress factors. In addition, in vitro maintenance of the collection allows the physiological, biochemical and molecular biological studies of Antarctic plants without damaging their natural population.		Interest in the study of Antarctic plants is associated with extreme conditions of this region and the influence of complex abiotic stress factors on their growing. Plants growing in vitro can serve as a model on which one can study the effect of stress factors. In addition, in vitro maintenance of the collection allows the physiological, biochemical and molecular biological studies of Antarctic plants without damaging their natural population.

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 Logistic and financial support of National Antarctic Scientific Center of Ukraine
 [[uk:Мохи Антарктики та Субантарктики in vitro]]
 [[ru:Мхи Антарктики и Субантарктики in vitro]]

← Older revision		Revision as of 12:42, 8 March 2015
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		+	'''[[Nadiia Matvieieva]]''' [[Laboratory of Adaptative Biotechnology]]
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	Interest in the study of Antarctic plants is associated with extreme conditions of this region and the influence of complex abiotic stress factors on their growing. Plants growing in vitro can serve as a model on which one can study the effect of stress factors. In addition, in vitro maintenance of the collection allows the physiological, biochemical and molecular biological studies of Antarctic plants without damaging their natural population.		Interest in the study of Antarctic plants is associated with extreme conditions of this region and the influence of complex abiotic stress factors on their growing. Plants growing in vitro can serve as a model on which one can study the effect of stress factors. In addition, in vitro maintenance of the collection allows the physiological, biochemical and molecular biological studies of Antarctic plants without damaging their natural population.

← Older revision		Revision as of 06:09, 4 April 2013
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	Logistic and financial support of National Antarctic Scientific Center of Ukraine		Logistic and financial support of National Antarctic Scientific Center of Ukraine
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		+	[[uk:Мохи Антарктики та Субантарктики in vitro]]
		+	[[ru:Мхи Антарктики и Субантарктики in vitro]]

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 [[Image:Samples from Antarctic peninsula 2011.jpg|600px]]
+[[Image:Samples from Galindez Island 2011.jpg|300px|right]]
 In vitro multiplication of mosses differed markedly. Growth of shoots of No16-2011, 12-2011,1-2011,3-2011, 19-2011 began in 3-10 days. Shoot growth of samples 6-2011, Ck2-2011, 2-2011, 18-2011 was not detected. However formation of strongly branched protonema was observed.  Protonema was sprouted or not into agarized semisolid medium.

← Older revision		Revision as of 20:31, 3 April 2013
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	Methods of surface sterilization and in vitro micropropagation of different Antarctic mosses were optimized. Most species of mosses from Antarctic islands and Antarctic Peninsula were very sensible to the conditions of surface sterilization.		Methods of surface sterilization and in vitro micropropagation of different Antarctic mosses were optimized. Most species of mosses from Antarctic islands and Antarctic Peninsula were very sensible to the conditions of surface sterilization.
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		+	{{-}}
		+	[[Image:Samples from Antarctic peninsula 2011.jpg\|600px]]
		+	{{-}}

	In vitro multiplication of mosses differed markedly. Growth of shoots of No16-2011, 12-2011,1-2011,3-2011, 19-2011 began in 3-10 days. Shoot growth of samples 6-2011, Ck2-2011, 2-2011, 18-2011 was not detected. However formation of strongly branched protonema was observed. Protonema was sprouted or not into agarized semisolid medium.		In vitro multiplication of mosses differed markedly. Growth of shoots of No16-2011, 12-2011,1-2011,3-2011, 19-2011 began in 3-10 days. Shoot growth of samples 6-2011, Ck2-2011, 2-2011, 18-2011 was not detected. However formation of strongly branched protonema was observed. Protonema was sprouted or not into agarized semisolid medium.

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 [[Image:Samples from Deception Island 2011.jpg|300px|left]]
 [[Image:Samples from Weddel Islands 2011.jpg|300px|left]]
 Research work on creation of in vitro collection of the representatives of Antarctic flora has been started in 2009. Now (in April 2011) the collection includes 34 specimens (vascular plants and mosses).

← Older revision		Revision as of 20:27, 3 April 2013
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	[[Image:Samples from Deception Island 2011.jpg\|300px\|left]]		[[Image:Samples from Deception Island 2011.jpg\|300px\|left]]
		+	[[Image:Samples from Weddel Islands 2011.jpg\|300px\|left]]
	Research work on creation of in vitro collection of the representatives of Antarctic flora has been started in 2009. Now (in April 2011) the collection includes 34 specimens (vascular plants and mosses).		Research work on creation of in vitro collection of the representatives of Antarctic flora has been started in 2009. Now (in April 2011) the collection includes 34 specimens (vascular plants and mosses).

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 [[Image:Samples from Peterman Island 2011.jpg|300px|left]]
 The collection of plants includes vascular plants and mosses from wide area of Antarctic islands and from continental Antarctica. These plant samples were collected from 29.12.2010 to 05.02.2011 on Antarctic Peninsula (Brown Bluff, Prospect Point Station) and on islands of Galindez, Peterman, Deception, Weddell, Barrientos, and Ushuaia.
 Plant shoots were surface sterilized under routine procedure (10 sec 70% ethanol; 1- 5 min “Belizna”; 60 min sterile H<sub>2</sub>O). The shoots were transferred to 1/2MS medium (Murashige, Skoog, 1962) and cultivated in in vitro conditions (16-hour light photoperiod, 24C) with subcultivation every two weeks.
 Research work on creation of in vitro collection of the representatives of Antarctic flora has been started in 2009. Now (in April 2011) the collection includes 34 specimens (vascular plants and mosses).