WikiSysop: /* Selected Publications */

2025-05-05T09:44:11Z

‎Selected Publications

WikiSysop at 09:40, 5 May 2025

2025-05-05T09:40:26Z

WikiSysop: Redirecting to Yakiv Ivanovych Ratushnyak

2010-10-18T15:07:27Z

Redirecting to Yakiv Ivanovych Ratushnyak

WikiSysop: New page: ==Scientific Interests== * organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants, * genetic variability of fertile asymmetric somat...

2009-03-31T20:08:28Z

New page: ==Scientific Interests== * organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants, * genetic variability of fertile asymmetric somat...

New page

==Scientific Interests==

* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
* genetic variability of fertile asymmetric somatic hybrids,
* plant genetic transformation.

===Experience===

* Plant cell tissue and protoplast culture,
* mutagenesis and mutant selection,
* haploid production,
* plant genetic transformation
** ''Agrobacterium''-mediated procedure,
** direct gene transfer with and without electroporation,
** chloroplast transformation,
* somatic hybridization,
* biochemical and molecular analyses
** DNA-fragment amplification by PCR,
** isozyme analysis.

← Older revision		Revision as of 09:44, 5 May 2025
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	[[Category:Staff\|Ratushnyak]]		[[Category:Staff\|Ratushnyak]]
		+	[[Category:Staff of the Department of genetic engineering\|Ratushnyak]]
		+	[[Category:Staff of the Laboratory of Adaptative Biotechnology]]
		+	[[Category:Yakiv Ratushnyak]]

	[[uk:Ратушняк Яків Іванович]]		[[uk:Ратушняк Яків Іванович]]
−	~~[[ru:Ратушняк Яков Иванович]]~~

← Older revision		Revision as of 09:40, 5 May 2025
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−	~~#REDIRECT~~ [[Yakiv Ivanovych Ratushnyak]]	+	[[Image:Yakiv Ivanovych Ratushnyak 2011.jpg\|400px\|left]]
		+	===M.Sc., Ph.D===
		+	===Date & Place of birth===
		+	April 5, 1961; Studena, Vinnytsya distr., Ukraine
		+
		+	==Contacts==
		+	{\|
		+	\|phone:\|\|+380 (44) 266-70-53
		+	\|-
		+	\|fax:\|\|+380 (44) 252-17-86
		+	\|}
		+	<!-- e-mail: rudas@rudas.ambernet.kiev.ua -->
		+
		+	==Education & Degrees==
		+	{\|
		+	\|\|1981-1986\|\|M.Sc. in Genetics\|\|Thesis: “Genetic complementation of tomates in somatic and sexual hybridization” Kyiv State University, Kyiv, Ukraine
		+	\|-
		+	\|1986-1991\|\|Ph.D. in Genetics.\|\|Thesis: “Transgenomic plants in <I>Lycopersicon</I> obtained by γ-hybridization of protoplasts” Institute of Cell Biology & Genetic Engineering Academy of Sciences of the Ukraine, Kyiv, Ukraine
		+	\|}
		+
		+	==Positions Held==
		+	{\|
		+	\|1997- present\|\|Senior Researcher\|\|Laboratory of Cytophysiology Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|-
		+	\|1997-1998\|\|Postdoctoral Fellow\|\|Laboratory of Prof. Dr. Peter Meyer University of Leeds, Leeds, United Kingdom
		+	\|-
		+	\|1991-1996\|\|Researcher\|\|Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|-
		+	\|1989-1991\|\|Research Assistant\|\|Laboratory of Cytophysiology & Cell Engineering Institute of Cell Biology & Genetic Engineering National Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|-
		+	\|1986-1989\|\|Research Assistant\|\|Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|-
		+	\|1983-1986\|\|Research Technician\|\|Laboratory of Cytophysiology & Cell Engineering Institute of Botany National Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|}
		+
		+	===Teaching experience===
		+	{\|
		+	\|1987-1990\|\|Teaching Assistant\|\|Principles of Tomato Cell Engineering\|\|Institute of Cell Biology & Genetic Engineering Academy of Sciences of Ukraine, Kyiv, Ukraine
		+	\|-
		+	\|1992-1997\|\|Teacher\|\|Plant Tissue Culture and Micropropagation\|\|Department of Cell Biology & Genetic Engineering, National University of Kyiv, Kyiv, Ukraine
		+	\|}
		+
		+	===Grants===
		+	{\|
		+	\|1993\|\|Individual Grant from the International Scientific Fund, USA
		+	\|-
		+	\|1994\|\|Travel Grant from the International Scientific Fund, USA
		+	\|-
		+	\|1997\|\|Posdoctoral Fellowship from the Royal Society/NATO, UK
		+	\|}
		+
		+	==Summary of Research Activities==
		+
		+	As a MSc student, I worked on protoplast culture and plant regeneration of <I>Lycium barbarum</I> (woody bush).
		+
		+	My PhD thesis was devoted to obtaining and analysing transgenomic plants of: <I>L. esculentum</I> with<I> L. peruvianum</I> var. <I>dentatum</I>, <I> L. pennellii</I>, <I>L. hirsutum</I> var. <I>glabratum,</I> <I>Solanum rickii </I>and <I>Lycium barbarum</I>. The results obtained and perspective of future investigations of fertile asymmetric somatic hybrids <I>L. esculentum</I> + <I>L. peruvianum</I> var. <I>dentatum</I> were announced in Rice Biotechnology Quarterly (Gamma Fusion of Tomato Protoplasts, 1991, V 8, p 38-39).
		+
		+	My postdoctoral work started with studies of tomato cybrid plants with chloroplasts of<I> L. peruvianum</I> var. <I>dentatum</I> and reconstructed mitochondria with phenotypic traits of nucleo-cytoplasmic incompatibility.
		+
		+	Then cybrid plants of <I>L. peruvianum</I> var. <I>dentatum</I> were obtained containing tomato chloroplasts and <I>L. peruvianum</I> var. <I>dentatum </I>mitochondria that along with cybrid tomato/<I>peruvianum</I> could serve as an excellent model for studies of nuclear-cytoplasmic and chloroplast-mitochondrial interactions.
		+
		+	Also, by restriction analysis of chloroplast DNA for asymmetric somatic hybrids between <I>L. esculentum</I> and <I>L. peruvianum</I> var. <I>dentatum</I> the presence of the new nonparental patterns was shown. Blot-hybridization of chloroplast DNA with <I>Nicotiana tabacum</I> chloroplast DNA fragments revealed rearrangements which might be interpreted as recombination of chloroplast DNA.
		+
		+	At the same time, for fertile asymmetric somatic hybrids <I>L. esculentum</I> + <I>L. peruvianum</I> var. <I>dentatum </I>a considerable genetic variability was shown. Morphological, cytogenetic, isozyme, ribosomal DNA and random amplified polymorphism DNA analyses of F1 and F2 progenies obtained after self-pollination of somatic hybrids revealed new nonparental patterns of DNA and enzymes, chromosome elimination and variation of leaf morphology.
		+
		+	Also, in collaboration with Prof. C.M.Rick (Tomato Stock Center, Davis, USA) we studied protoplast culture of other species of <I>Petota </I>section <I>S. ochrantum</I>, <I>S. juglandifolium</I>, as well as possibilities of their somatic hybridization with tomato.
		+
		+	Then I worked on genetic transformation of <I>Arabidopsis</I> by leaf-disk and root co-cultivation with <I>Agrobacterium </I>in a project<I> </I> named “Identification, isolation and analysis of stress-induced plants genes in <I>Arabidopsis thaliana</I> by T-DNA tagging and gene fusion” funded by the INTAS. <I> </I>
		+
		+	Also, I worked on obtaining haploid plants from <I>Petunia </I>cultivars and <I>Nicotiana</I> species (using anther and pollen culture). These are used in activation tagging experiments aimed to achieve genetic transformation by co-cultivation of haploid protoplasts with <I>Agrobacterium</I>. Co-cultivation allowed the selection some PEG- and salt-resistant clones of N. tabacum. These clones might become useful material to isolate salt- and PEG-resistant plants, and to identify genes responsible for salt- and PEG-resistant. I also study possibilities of the use of diploid protoplasts of <I>Arabidopsis thaliana</I> in these experiments. The title of the project is “Activation tagging <I>Arabidopsis thaliana</I> and<I> Petunia hybrida” </I>and it is<I> </I>funded by the Royal Society and NATO.
		+
		+	At present, I am studying possibilities for successful integration of foreing sequences into plastome of some Solanaceae species via PEG-mediated protoplast transformation.
		+
		+	==Scientific Interests==
		+	* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
		+	* genetic variability of fertile asymmetric somatic hybrids,
		+	* plant genetic transformation.
		+
		+	===Experience===
		+	Plant cell tissue and protoplast culture, mutagenesis and mutant selection, haploid production, plant genetic transformation (<I>Agrobacterium</I>-mediated procedure, direct gene transfer with and without electroporation, chloroplast transformation), somatic hybridization, biochemical and molecular analyses (DNA-fragment amplification by PCR, isozyme analysis).
		+
		+	==Selected Publications==
		+	# Ratushnyak YI, Piven NM, Rudas VA (1989) Protoplast culture and plant regeneration in <I>Lycium barbarum</I> L. Plant Cell Tissue Organ Cult 17:183-190</P>
		+	# Ratushnyak YI, Piven NM, Rudas VA (1990) Regeneration of <I>Lycium barbarum</I> L. plants from leaf tissue, callus culture and callus protoplasts. Plant Cell Rep 9:84-87</P>
		+	# Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybrydization of tomatoes by "gamma-fusion" of the <I> Lycopersicon esculentum </I>Mill. and <I>Lycopersicon peruvianum</I> var. <I>dentatum</I> Dun. protoplasts. Plant Sci 73:65-78</P>
		+	# Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba YY (1991) Changes of the genetic constitution of the tomato plants by 'gamma-fusion' of <I>Lycopersicon esculentum</I> Mill. and <I>Lycopersicon peruvianum</I> var. <I>dentatum</I> Dun. protoplasts. Biopolymers and Cell (Rus) 7:82-91</P>
		+	# Ratushnyak YI (1991) Gamma fusion of tomato protoplasts. Rice Biotechnology Quarterly 8:38-39</P>
		+	# Ratushnyak YI, Rudas VA, Piven NM (1993) Regeneration of plants from protoplasts of <I>Lycium barbarum</I> L. (Wolfberry). In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol 22, Plant Protoplasts and Genetic Engineering III, Springer, New York, pp 79-86</P>
		+	# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Fertile asymmetric somatic hybrids between <I>Lycopersicon esculentum</I> Mill. and <I>Lycopersicon peruvianum</I> var. <I>dentatum</I> Dun. Mol Gen Genet, 236: 427-432</P>
		+	# Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993) Cell engineering synthesis of tomato fertile asymmetric somatic hybrids by "fusion" of protoplasts. Genetics (Russia), 29:164-176</P>
		+	# Ratushnyak YI, Cherep NN, Latypov SA (1994) Somatic hybridization of <I>Lycopersicon esculentum</I> Mill. + <I>Lycopersicon peruvianum</I> var. <I>dentatum</I> Dun. In: Bajaj YPS (ed) Biotechology in Agriculture and Forestry, Vol. 27 Somatic Hybridization in Crop Improvement, Springer, New York, pp 224-243</P>
		+	# Ratushnyak YI, Kochevenko AS, Cherep NN, Zavgorodnyaya AV, Latypov SA, Gleba YY (1995) Alloplasmatic incompatibility in cybrid plants possessing <I>Lycopersicon esculentum</I> Mill. genome and <I>Lycopersicon peruvianum</I> var. <I>dentatum</I> Dun. plasmagenes. Russian Journal of Genetics, 31:565-571</P>
		+	# Kochevenko AS, Ratushnyak YI, Gleba YY (1996) Protoplast culture and somaclonal variability of species of series<I> Juglandifolia</I>. Plant Cell Tissue Organ Cult, 44:103-110<I> </I> </P>
		+	# Kochevenko AS, Rudas VA, Ratushnyak YI (1999) Analysis chlorophyll-deficiency mutation of <I>Lycopersicon peruvianum</I> var.<I> dentatum</I> by obtain the cytoplasmic hybrids. Russian Journal of Genetics 35:1-8</P>
		+	# Kochevenko AS, Ratushnyak YI, Korneev DU, Stasik OO, Shevchenko VV, Kochubey SM, Gleba YY (1999) Photosynthetic apparatus in cytoplasmic hybrid of cultured tomato carrying nuclear-cytoplasmic incompatibility traits. Russian Journal of Plant Physiology, 46:474-481 </P>
		+
		+	[[Category:Staff\|Ratushnyak]]
		+
		+	[[uk:Ратушняк Яків Іванович]]
		+	[[ru:Ратушняк Яков Иванович]]

@@ Line 1: / Line 1: @@
-==Scientific Interests==
+#REDIRECT [[Yakiv Ivanovych Ratushnyak]]
-−
-* organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants,
-* genetic variability of fertile asymmetric somatic hybrids,
-* plant genetic transformation.
-−
-===Experience===
-−
-* Plant cell tissue  and  protoplast  culture,
-* mutagenesis  and  mutant  selection,
-* haploid production,
-* plant genetic transformation
-** ''Agrobacterium''-mediated procedure,
-** direct gene transfer with and without electroporation,
-** chloroplast transformation,
-* somatic hybridization,
-* biochemical and molecular analyses
-** DNA-fragment amplification by PCR,
-** isozyme analysis.

Yakiv Ratushnyak - Revision history

WikiSysop: /* Selected Publications */

WikiSysop at 09:40, 5 May 2025

WikiSysop: Redirecting to Yakiv Ivanovych Ratushnyak

WikiSysop: New page: ==Scientific Interests== * organelle DNA recombination and nucleo-cytoplasmic interactions under somatic hybridization in higher plants, * genetic variability of fertile asymmetric somat...