Dmytro Yevtushenko

Research experience:

Plant cell and tissue culture:

development of new protocols for plant regeneration from protoplasts and organ tissues, induction of callus and suspension culture in vitro, isolation and cultivation of protoplasts, microclonal propagation, protoplast fusion, mutagenesis and selection procedures.

Transformation of plants:

Agrobacterium-mediated (including floral dip method), direct gene transfer by microprojectile bombardment, electroporation of protoplasts, polyethylene glycol-mediated, etc.

Plant molecular biology:

cDNA library screening, gene cloning, construction of all types of vectors for transient and stable (trans)gene expression in bacterial, plant, and animal systems, analyses of spatiotemporal promoter activity in vivo using GFP and GUS reporter gene system, identification and deletion analyses of regulatory elements in promoters, isolation and Southern analyses of genomic, chloroplast and mitochondrial DNA, design and synthesis of PCR primers, conventional PCR, RACE-PCR, analyses of gene expression (northern blotting, differential display, RT-PCR, etc.) and protein accumulation (AU-PAGE, western blotting and immunodetection, histochemical localization), detection of enzyme activity (e.g. fluorometric GUS assay), isoenzyme analyses.

Assessment of disease resistance in plants under field conditions and in vitro.

Methods of cytogenetic analysis.

Education and degrees:

1996	Ph.D. in Cell Biology, Institute of Cell Biology & Genetic Engineering, Kiev, Ukraine. Dissertation title: “Production and analysis of interspecific cytoplasmic hybrids of potato”. Advisor: Dr. Vladimir Sidorov.
1991-1996	Postgraduate (Ph.D.) student course at the Institute of Cell Biology & Genetic Engineering, Kiev, Ukraine.
1991	M.Sc. in Plant Physiology and Biochemistry (with Distinction), Kiev State University, Ukraine. Thesis title: "Mutagenesis of chloroplast DNA and production of potato lines resistant to streptomycin”. Advisor: Dr. Vladimir Sidorov.
1984-1991	Undergraduate and graduate courses at the Department of Biology: Division of Physiology and Biochemistry of Plants. Kiev State University, Ukraine.

Supervised and mentored:

Twelve graduate students and junior scientists

University courses taught:

• Cell Selection
• Plant Biotechnology

Invited reviewer for:

• Plant Biotechnology Journal
• Planta.

Professional membership:

• 1996-present Canadian Society of Plant Physiologists (CSPP)
• 2005-present Advanced Foods and Materials Network (AFMNet)