Nataliia Kutsokon

Nataliia Kutsokon
PhD
Senior Scientist

Address: Institute of Cell Biology and Genetic Engineering of Ukrainian National Academy of Sciences, Acad. Zabolotnogo str., 148, Kyiv, UKRAINE, 03143
TEL.: (+ 038044) 257-82-44
FAX: (+38044) 258-10-50
E-mail: kutsokon@nas.gov.ua

SCIENTIFIC INTERESTS:

Gene engineering, fast-growing trees, genetic transformation of plants, Populus sp., plants in vitro cultivation, stress tolerance, mutagenesis, cytogenetics, biotesting, antimutagenesis, environmental mutagenesis, ageing

EDUCATION:

2005 – PhD (Biology, Radiobiology) degree obtaining
2000 – 2003 PhD Studentship. Institute of Cell Biology and Genetic Engineering of Ukrainian National Academy of Science
1991 - 1997 Kyiv State University, Faculty of Biology, M.Sc. in Genetics

CAREER/EMPLOYMENT

2003 - present – Institute of Cell Biology and Genetic Engineering of Ukrainian National Academy of Sciences, Department of Biophysics and Radiobiology, Kyiv - Junior Scientific Researcher, Senior Scientist
2005 – 2009 – National Agriculture University, Department of Molecular Genetics and Biosafety, Kyiv - Assistant of Professor, Associated Professor
1997 – 2000 – Ukrainian Scientific Center of Medical Genetics Ukrainian Academy of Medical Sciences, Department of Mutagenesis and Antimutagenesis, Kyiv - Junior Scientific Researcher

RESEARCH EXPERIENCE:

Plants in vitro cultivation, plant transformation, bacterial cloning, DNA isolation, PCR, plant cytogenetic assays, Tradescantia-SH assay, microscope technique, computer skills

TEACHING EXPERIENCE:

Full courses of lectures in Genetics and Applied Genetics with Principles of Cytology at National Agriculture University of Ukraine. Supervisor of students’ Bachelor and Master Diplomas

INTERNATIONAL TRANINGS/COURSES:

• Comet-assay studying, Dr. Tomáš Gichner, Institute of Experimental Botany, Academy of Sciences of Czech Republic, Prague, May, 2007
• Training course "Molecular Aspects of Salt and Drought Tolerance in Crops (plants)", Genetic Engineering Research Center (GERC), Faculty of Agriculture, Cairo University, Egypt, September, 2007
• CIMO Winter School “Regulatory Systems: From Basic Research to Disease Prevention”, Finland, April, 2008. (Supported by CIMO travel grant)
• Short-training on tree breeding and the tolerance of trees against stresses, Dr. Pertti Pulkkinen, Finnish Forest Research Institute, METLA's Haapastensyrjä Breeding Station, Finland, April, 2008
• Training on “Generation of transgenic plants and verification of their transgenic nature”, Dr. Jana Libantova, Institute of Plant Genetics and Biotechnology, Nitra, Slovak Republic, April – August, 2009 (Supported by grant of National Scholarship Program of the Slovak Republic for the support of Mobility of students, PhD students, university teachers and researchers)
• NATO Advanced Training Course on “Rapid Diagnosis in Population at Emergency and Risk “RADIPER”. Krakow-Zakopane, Poland, 19-23 October, 2009 (Supported by NATO travel grant)
• Training on “Establishing the protocol for Drosera transformation. Verification of putative transgenic plants of Populus transformed by AV04 gene construct”, Dr. Jana Libantova, Institute of Plant Genetics and Biotechnology, Nitra, Slovak Republic, 1 April – 31 August, 2011 (Supported by grant of National Scholarship Program of the Slovak Republic for the support of mobility of students, PhD students, university teachers and researchers)
• Fulbright Visiting Scholar, project ”Advancing the methods for planting and creating new clones of poplars for short-rotation forests”, Prof. Jose Shibu, Center for Agroforestry, University of Missouri, Columbia, USA, 4 February – 3 November 2013
• The Swedish Institute Visby Programme 2017/2018 for postdoctoral researchers and senior scientists, project “Productivity of fast growing forest trees in the lights of global climate changes”, Prof. Martin Weih, Swedish University of Agricultural Sciences, Uppsala, Sweden, 01 January – 30 June 2018

AWARDS

2006 –	Award of Kiev city administration for young scientists
2009 -	A Prize of the Parliament of Ukraine for the young researchers for scientific excellence

PROFESSIONAL MEMBERSHIPS:

• Ukrainian Biochemical Society
• Ukrainian Society of Geneticists and Breeders
• Radiobiological Society of Ukraine

INTERNATIONAL PUBLICATIONS

• Kryvokhyzha M., Litvinov S., Danchenko M., Khudolieieva L., Kutsokon N., Baráth P., Rashydov N. (2024) How does ionizing radiation affect amyloidogenesis in plants?, International Journal of Radiation Biology, [[1]]
• Kutsokon N., Rakhmetov D., Rakhmetova S., Khudolieieva L., Rashydov N. (2022) Nursery screening of poplar and willow clones for biofuel application in Ukraine. iForest: Biogeosciences and Forestry, 15(5):401-410. [[2]]
• Khoma Y., Khudolieieva L., Rashydov N., Kutsokon N. (2022) In vitro culture initiation and regeneration of two highly productive clones of poplar. Nova Biotechnologica et Chimica, 21(1):e1089. [[3]]
• Khoma Y.A., Nesterenko O.G., Kutsokon N.K., Khudolieieva L.V., Shevchenko V.V., Rashydov N.M. (2021). Proline content in the leaves of poplar and willow under water deficit. Regulatory Mechanisms in Biosystems, 12(3):519-522. [[4]]
• Kutsokon, N., Danchenko, M., Skultety, L., Kleman, J., Rashydov N. (2020) Transformation of hybrid black poplar with selective and reporter genes affects leaf proteome, yet without indication of a considerable environmental hazard. Acta Physiologia Plantarum, 42:86. [[5]]
• Kutsokon N.K., Jose S., Holzmueller E. (2015) A global analysis of temperature effects on Populus plantation production potential.American Journal of Plant Sciences,6(1):23-33. [[6]]
• Kutsokon N., Libantova J., Rudas V., Rashydov N., Grodzinsky D., Ďurechová D. (2013) Advancing protocols for poplar in vitro propagation, regeneration and selection of transformants // Journal of Microbiology, Biotechnology and Food Sciences. 2 (Special issue):1447-1454.[[7]]
• Kutsokon N. K. (2011) Main Trends in the genetic transformation of Populus species. Cytology and Genetics, Allerton Press, Inc. 45(6):352–361. (In English).[[8]]
• Kutsokon N.K. (2011) Main Trends in Genetic Transformation of Populus. Cytology and Genetics, 6:67-78. (In Ukrainian)
• Kolodyazhnaya Y.S., Kutsokon N.K., Levenko B.O. Syutikova O.S., Rakhmetov D.B., Kochetov A.V. (2009) Transgenic plants, tolerant to abiotic stresses. Cytology and Genetics, 43(2):72-O3.[[9]]
• Kutsokon N.K. (2008) Allium -assay in evaluation of drinking and surface water mutagenicity. In: Dangerous Pollutants (Xenobiotics) in Urban Water Cycle. Eds. Hlavinek, P.; Bonacci, O.; Marsalek, J.; Mahrikova, I. Springer, 81-87.[[10]]
• Kutsokon N.K., Rashydov N.M., Grodzinsky D.M. (2008) Radiation induction of multiaberrant cells in Allium cepa L. Proceedings of International Conference on Radioecology and Environmental Radioactivity, Norway, Bergen, 15-20 June, 271-277.
• Rashydov N.M., Berezhna V.V., Kutsokon N.K. (2007) Evaluation of water and soil genotoxicity with bioassays in sites contaminated with radionuclides. In: Proceedings of MEDA WATER International Conference, Sustainable Water Management. Tunis, 21-24 March, 307-312.
• Rashydov N.M., Berezhna V.V., Kutsokon N.K. (2007) Risk assessment of wastewater contaminated with radionuclides using Arabidopsis thaliana L. plants. In: Wastewater Reuse Risk Assessment, Decision-making and Environmental Security NATO Advanced Research Workshop on Wastewater Reuse (2006: Istanbul, Turkey) Ed. Mohammed K. Zaidi. Springer, 121-130.
• Kutsokon N.K., Lazarenko L.M., Bezrukov V.F., Rashydov N.M., Grodzynsky D.M. (2004) The number of aberrations in cells as a parameter of chromosomal instability. 2. Comparative analysis of effects induced by different mutagenes. Cytology and Genetics 38(1):49-56.
• Kutsokon N., Rashidov N., Berezhna V., Grodzinsky D.M. (2004) Biotesting of radiation pollutions genotoxicity with the plants bioassays. In: Radiation safety problems in the Caspian region. Kluwer Academic Publishers, 51-56.
• Kutsokon N.K., Bezrukov V.F., Lazarenko L.M., Rashydov N.M., Grodzynsky D.M. (2003) The number of aberrations in aberrant cells as a parameter of chromosomal instability. 1. Characterization of dose dependency. Cytology and Genetics 37(4):17-23.

OTHER PUBLICATIONS

• Rakhmetov D., Bondarchuk O., Rakhmetova S., Rashydov N., Kutsokon N. (2023). Biological, morphological and biochemical features of seeds of introduced genotypes of Cicer arietinum L.. Plant and Soil Science, 14(3), 97-100. [[11]]
• Khoma Y.A., Khudolieieva L.V., Kutsokon N.K. (2020) Influence of salt stress on plants of poplar clone 'INRA 353-38' and willow clone 'Zhytomyrska – 1' in in vitro culture. Visnyk Taras Shevchenko National University of Kyiv. Biology, 83: 43-49.
• Kutsokon N.K, Khudolieieva L.V., Los S.A., Torosova L.O., Vysotska N.Y. (2020). Evaluation of poplar and willow clones on the experimental short rotation plantation in Kharkiv region: results of the second cultivation year. Plant Varieties Studying and Protection, 16(2):182–190. [[12]]
• Khoma Y.A., Kutsokon N.K. (2019) Bud burst phenology in different poplar and willow clones. Visnyk Taras Shevchenko National University of Kyiv. Biology, 79: 79-84.
• Khoma Y.A., Kutsokon N.K., Rashydov N.M., Pavlisky V.M., Nesterenko О.V. (2018) Effect of adding of glycerine on density of pellet from wheat straw and aspen wood. Scientific reports of NULES of Ukraine, 5(75).
• Khudolieieva L., Kutsokon N. (2018) In vitro evaluation of salt tolerance of poplars and willows. Science Rise: Biological Science, 11 (2): 35–38. [[13]]
• Kutsokon N.K., Khudolieieva L.V., Los S.A., Vysotska N.Y., Torosova L.O., Tkach V.P., Nesterenko O.G., Rashydov N.M. (2018) Evaluation of growth characteristics of one-year poplar and willow clones in short rotation plantation in Kharkiv region. Studia Biologica, 12(1): 55-64.
• Kutsokon N., Rakhmetov D., Khudolieieva L., Rakhmetova S., Fishchenko V., Nesterenko O., Rashydov N. (2017) Growth characteristics and energy productivity of poplars and willows under short rotation planting for the first vegetation year. Biological Systems, 9(2): 238-246.
• Khudolieieva L. V., Kutsokon N. K., Nesterenko O. G., Rashydov N. M., Dugan O. M. (2017) In vitro establishing of poplar and willow clones perspective for renewable energetics. Biological Systems, 9(1): 18–22.
• Kutsokon N.K., Rudas V.A., Shinkaruk M.V., Lakhneko O.R., Morgun B.V., Rashydov N.M., Grodzynsky D.M. (2016) Genetic transformation of Populus nigra x P. deltoides (black poplar, clone Gradizka). Visnyk UTGiS, 14(2): 187–191. [[14]]
• Khudolieieva L., Kutsokon N., Rashydov N., Dugan O. (2016) Quantitative and qualitative evaluations of environmentally dangerous wastes emission from burning wood comparing to natural gas and coal. Studia Biologica, 10(3): 61-70. [[15]]
• Khudoleeva L.V., Kutsokon N.K., Nesterenko O.G., Rudas V.A., Rashydov N.M., Grodzynsky D.M., Dugan O.M., Bulbotka K.S. (2014) Microclonal propagation for creation of fast-growing poplar plantations for alternative energy needs. Visnyk UTGiS, 12(2): 226–233.
• Kutsokon N.K. (2014) Thirteen interesting facts about poplar. Biology and Chemistry in the School, 5: 45-47.
• Kutsokon N.K. (2010) Plant biotests for evaluation of genotoxicity. Visnyk NASU, 4:48 – 52.
• Kutsokon N.K., Rashydov N.M. (2008) Effects of chronic irradiation of plants induced by technogenic radionuclides. Radiobiological effects of chronic irradiation in plants in Chornobyl accident zone. Ed. D.M. Grodzinsky. Kyiv: Naukova Dumka, NAS Ukraine. p. 70–126.
• Grodzinsky D.M., Shylina Y.V., Kutsokon N.K., Mikheev O.M., Gushcha M.I., Kolomiyets O.D., Falinska T.P., Ovsyannikova L.G., Kutlakhmedov Y.O., Pchelovska S.V. (2006) Using the plant bioassays in evaluation of combined effects of different factors: Recommendations for evaluation of permissible radionuclide and chemical pollution levels under combined action. Kyiv: Phytosociocenter.
• Rashydov N.M., Kutsokon N.K. (2005) Target and non-target radiobiological reactions – its threshold and non-threshold effects. Problems of Nuclear Power Plants Safety and of Chornobyl 3(2): 42–49.
• Kutsokon N.K., Neumerzhitska L.V., Kuznetsova G.M., Baryliak I.R. (2003) Effects of folic acid on level of spontaneous and induced by thioTEPA chromosome instability in Allium cepa cells. Problems of Ecological and Medical Genetics and Clinical Immunology 3 (49):76–88.
• Kutsokon N.K., Rashidov N.M., Grodzinskii D.M. (2002) Cytogenetic effects of ²⁴¹Аm in the Allium-test. Radiats Biol Radioecol 42(6): 675-677.
• Kutsokon N. K., Grodzinsky D. M., Rashidov N. M., Trishin V.V. (2002) Evaluation of ²⁴¹Аm genotoxicity in test-system of Allium cepa L. seedlings root tip cells. Scientific Papers of the Institute for Nuclear Research 17(1): 139-146.
• Barilyak I.R., Neumerzhitska L.V., Kutsokon N.K. (2001) Ecogenetics evaluation of recreation zones in Kyiv. Hygiene of Populated Sites 38(1): 97–100.
• Kutsokon N.K., Neumerzhitska L.V., Barilyak I.R. (2001) Cytogenetic effect of thioTEPA in Allium cepa L. root tip cells. Problems of Ecological and Medical Genetics and Clinical Immunology 5(37): 36-40.
• Kutsokon N.K., Neumerzhitska L.V., Barilyak I.R. (1999) Assessment of the genotoxicity of water samples taken from reservoirs in Kiev. Problems of Ecological and Medical Genetics and Clinical Immunology 2(22): 13-18.
• Kutsokon N.K., Lazarenko L.M., Bezrukov V.F. (1999) Evaluation of mutagenic activity of water from pipelines and reservoirs of Kyiv. Bulletin of Kiyv University. Biology 29: 28-30.

PATENTS

• Patent № 144268, Ukraine. The way of producing pellets from biomass of one-year paulownia shoots / Khoma Y.A., Rakhmetov D.B., Rakhmetova S.O., Fishchenko V.V., Nesterenko O.V., Kutsokon N.K., Rashydov N.M. Published 25.09.2020 in Bull. №18, 2020. 2 p.
• Patent № 13550, Ukraine. The way for improving quality of pellets from biomass / Khoma J.A., Kutsokon N.K., Rashydov N.M., Pavlisky V.M., Nesterenko O.V. Registered 10.07.2019. Published in Bull. №13, 2019. 2 p.
• Patent № 107853, Ukraine. The way for rooting poplar and willows cuttings / Kutsokon N.K, Vysotska N.Y., Torosova L.O., Los S.A., Rashydov N.M., Tkach V.P., Grodzinsky D. Registered 15.12.2015. Published in Bull. №12, 2016. 3 p.
• Patent № 58505, Ukraine. Approach for microclonal propagation the plants of Populus genus / Grodzinsky D., Rashydov N.M., Kutsokon N.K, Gushcha M.I., Shylina Y.V., Volosyanchuk R.T., Tkach V.P., Zolotykh I.V., Vysotska N.Y., Raksha-Slusareva О.А., Krul V.О. Registered 11.04.2011. Published in Bull. №7, 2011. 3 p.

RESEARCH PROJECTS

• The National Research Foundation of Ukraine, “Support for Research of Leading and Young Scientists”, the project “Effects of stress factors on prion-like proteins synthesis in plants” (2020-2023)
  
• FP7 Program People Marie Curie Actions International Research Staff Exchange Scheme (IRSES), the project “Plant adaptation to heavy metal and radioactive pollution” (2013-2017)
  
• Ukrainian-Slovak joint research project “The influence of the overexpression of сinnamyl alcohol dehydrogenase gene in transgenic poplar on the process of lignification” (2011-2013)
  
• Project supported by the National Academy of Sciences of Ukraine "Developing the gene engineering approaches for creating high productive poplar clones for biofuel production" (2010-2012)
  
• Project supported by the National Academy of Sciences of Ukraine Microclonal propagation and genetic transformation of Populus sp. for improving the abiotic stress tolerance (2007-2009)