Taras Pasternak
From ICBGE
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| − | + | == Research skills == | |
| + | ===Cellular biology skills:=== | ||
| + | * protoplast culture | ||
| + | * suspension culture | ||
| + | * cell cycle investigation | ||
| + | * plant tissue culture and transformation mediated by Agrobacterium tumefaciens or by biolistic bombardment | ||
| + | * RNA in situ hybridization | ||
| + | * free-hand sectioning and paraffin sectioning | ||
| + | * whole-mounting in situ immunolocalization | ||
| + | * laser scanning microscopy | ||
| + | * cell and tissue staining with fluorescent dyes | ||
| + | ===Molecular biology skill:=== | ||
| + | * plasmid construction | ||
| + | * DNA/RNA/protein extraction | ||
| + | * Southern, Northern and Western blotting | ||
| + | * PCR, RT-PCR, Semi-quantitative RT-PCR, Quantitative RTPCR and Tail-PCR | ||
| + | ===Bioinformatics skills:=== | ||
| + | * Sequence analysis | ||
| + | * High-throughput image analysis | ||
[[Category:Staff|Pasternak]] | [[Category:Staff|Pasternak]] | ||
Revision as of 11:18, 3 February 2013
Date of birth: March 27, 1959, Mukachevo, Ukraine
Nationality: Ukranian
Language skills: Russian, Ukrainian, English, German
Present address: Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany
E-mail: taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com
Contents |
Education
| 1983-1986 | Ph.D. study in the Institute of Botany, National Academy of Sciences of the Ukraine, Kiev, under supervision of Prof. Yu.Yu. Gleba. PhD thesis "Genetic transformation of higher plants by microinjection", Ph.D. degree obtained in April, 1987. |
| 1976-1981 | Graduate course in the Kharkov State University, Radiophysical Faculty, Department of Biophysics, Master of Science degree obtained, diploma work in the Institute of Low Temperature, Academy of Sciences of the Ukraine, Kharkiv. |
Positions held:
| 2004-2011 | Guest docent in the Institute of Biology II/Botany University of Freiburg. Project: «Crosstalk between auxin and ROS in the regulation of the Arabidopsis development» (2004-2011). |
| 2003 | Guest professor in the University of Antwerp. Project: «The role of ascorbate and glutathione in the regulation of plant growth and development» (2003). |
| 1998-2002 | Research fellow in Institute of Plant Biology, Hungarian Academy of Science, Szeged. Projects: «Fluorescent microscopy as a tools for studying the early events in cell division and somatic embryogenesis in alfalfa root and leaf protoplasts» (1998); «Role of oxidative stress-inducing agents in auxin-mediated and H2O2 -dependent activation of cell division in cultured plant cells» (1999-2000); «Physiological analysis of role of retinoblastoma gene in alfalfa and tobacco» (2001-2002); «Possible role of nitric oxide in cell cycle and embryogenesis regulation in cultured alfalfa cells» (2002). |
| 1997 | UNESCO fellowship Institute of Botany, Hamburg (1997). Project: «In vitro plant regeneration from mature cereal organs». |
| 1983-... | Research fellow in Institute of Cell Biology and Genetic Engineering, Kiev. Projects: «Genetic transformation of higher plants by microinjection» (1983-1987), «Genetic transformation of cereals» (1988-1994); «Chemical mutagenesis in cereals by mutagenic treatment of zygotic embryo cells» (1988-1994); «Molecular genetic analysis of oxidative stress in plants» (1994-1997). |
| 1981-1983 | Junior Research Fellow, Institute of Low Temperature, Kharkiv (1981-1983). |
Research skills
Cellular biology skills:
- protoplast culture
- suspension culture
- cell cycle investigation
- plant tissue culture and transformation mediated by Agrobacterium tumefaciens or by biolistic bombardment
- RNA in situ hybridization
- free-hand sectioning and paraffin sectioning
- whole-mounting in situ immunolocalization
- laser scanning microscopy
- cell and tissue staining with fluorescent dyes
Molecular biology skill:
- plasmid construction
- DNA/RNA/protein extraction
- Southern, Northern and Western blotting
- PCR, RT-PCR, Semi-quantitative RT-PCR, Quantitative RTPCR and Tail-PCR
Bioinformatics skills:
- Sequence analysis
- High-throughput image analysis
