https://icbge.org.ua/en/index.php?title=Taras_Pasternak&feed=atom&action=historyTaras Pasternak - Revision history2024-03-29T08:57:03ZRevision history for this page on the wikiMediaWiki 1.19.24https://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=253&oldid=prevWikiSysop at 17:41, 3 February 20132013-02-03T17:41:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Personal web site:''' [http://www.pasternak.pp.ua]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Personal web site:''' [http://<ins class="diffchange diffchange-inline">www.pasternak.pp.ua </ins>www.pasternak.pp.ua]</div></td></tr>
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</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=252&oldid=prevWikiSysop at 17:40, 3 February 20132013-02-03T17:40:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Personal web site:''' [www.pasternak.pp.ua]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Personal web site:''' [<ins class="diffchange diffchange-inline">http://</ins>www.pasternak.pp.ua]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Personal web site:''' www.pasternak.pp.ua</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Personal web site:''' <ins class="diffchange diffchange-inline">[</ins>www.pasternak.pp.ua<ins class="diffchange diffchange-inline">]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Language skills:''' Russian, Ukrainian, English, German<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Language skills:''' Russian, Ukrainian, English, German<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Personal web site:''' www.pasternak.pp.ua</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Personal web site:''' www.pasternak.pp.ua</div></td></tr>
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</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=249&oldid=prevWikiSysop at 11:51, 3 February 20132013-02-03T11:51:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Language skills:''' Russian, Ukrainian, English, German<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Language skills:''' Russian, Ukrainian, English, German<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<del class="diffchange diffchange-inline"><br clear=all></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Personal web site:''' www.pasternak.pp.ua</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Personal web site:''' www.pasternak.pp.ua</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Education==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Education==</div></td></tr>
</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=248&oldid=prevWikiSysop at 11:51, 3 February 20132013-02-03T11:51:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Present address:''' Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br clear=all></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''E-mail:''' taras.pasternak@biologie.uni-freiburg.de or paster59@yahoo.com<br clear=all></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Education==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Education==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Publications and Citations==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Publications and Citations==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://scholar.google.com/citations?hl=en&user=kWbssy8AAAAJ&view_op=list_works On Google Scholar]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://scholar.google.com/citations?hl=en&user=kWbssy8AAAAJ&view_op=list_works On Google Scholar]</div></td></tr>
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</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=247&oldid=prevWikiSysop at 11:47, 3 February 20132013-02-03T11:47:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Regulator of the cells polarity===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Regulator of the cells polarity===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The methods we developed have been also applied to investigation of the mechanism of the cell polarity induction in the Arabidopsis thaliana. Our data demonstrate for the first time that BFA at low dose (1 to 3 μM) have strong impact on auxin distribution, root growth, root apical meristem architecture and stem cells maintenance independently from internalizations of the membrane proteins.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The methods we developed have been also applied to investigation of the mechanism of the cell polarity induction in the Arabidopsis thaliana. Our data demonstrate for the first time that BFA at low dose (1 to 3 μM) have strong impact on auxin distribution, root growth, root apical meristem architecture and stem cells maintenance independently from internalizations of the membrane proteins.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[http://scholar.google.com/citations?hl=en&user=kWbssy8AAAAJ&view_op=list_works On Google Scholar]</ins></div></td></tr>
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</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=231&oldid=prevWikiSysop: /* Education */2013-02-03T08:32:45Z<p><span dir="auto"><span class="autocomment">Education</span></span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|1983-1986|| Ph.D. study in the Institute of Botany, National Academy of Sciences of the Ukraine, Kiev, under supervision of Prof. Yu.Yu. Gleba. PhD thesis "Genetic transformation of higher plants by microinjection", Ph.D. degree obtained in April, 1987.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|1983-1986|| Ph.D. study in the Institute of Botany, National Academy of Sciences of the Ukraine, Kiev, under supervision of Prof. <ins class="diffchange diffchange-inline">[[</ins>Yu.Yu. Gleba<ins class="diffchange diffchange-inline">]]</ins>. PhD thesis "Genetic transformation of higher plants by microinjection", Ph.D. degree obtained in April, 1987.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1976-1981|| Graduate course in the Kharkov State University, Radiophysical Faculty, Department of Biophysics, Master of Science degree obtained, diploma work in the Institute of Low Temperature, Academy of Sciences of the Ukraine, Kharkiv.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|1976-1981|| Graduate course in the Kharkov State University, Radiophysical Faculty, Department of Biophysics, Master of Science degree obtained, diploma work in the Institute of Low Temperature, Academy of Sciences of the Ukraine, Kharkiv.</div></td></tr>
</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=230&oldid=prevWikiSysop at 08:21, 3 February 20132013-02-03T08:21:08Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* High-throughput image analysis</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* High-throughput image analysis</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Research interests==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Investigation of the interaction between environmental factors, plant architecture and hormonal system of Medicago sativa L. and Arabidopsis thaliana L.'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Together with my co-workers we have developed several methods to trace gene expression and organ morphology at different levels. These methods include:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* isolation and cultivation of the isolated protoplasts from root and leaf with further possibilities of direct embryogenesis;</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* whole mounting molecules immune-localization at whole seedlings, organs and cellular level with further 3D organ/cells reconstruction</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* creating virtual 3D nucleus map of Arabidopsis roots;</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">All these protocols have been used as tools to further investigation of interaction of environmental factors, low molecular weight antioxidants, inhibitor of cell polarization Breferldin A with hormonal system of Arabidopsis thaliana and Medicago sativa.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Low molecular weight antioxidant===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We investigated interaction between GSH level and hormonal system by using as examples Arabidopsis thaliana root system and Medicago sativa organs and single cells.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The resulting data demonstrate for the first time that GSH impacts on auxin mediated responses as well as auxin transporters localization, hence serving as an auxin-response modulator. In the Medicago sativa we have found that hGSH have a different role than GSH: GSH involvement in the regulation of cell division, while hGSH play a significant role in the cell differentiation.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Reactive oxygen species===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Next, we investigated interaction of reactive oxygen species with auxin in the regulation of root development in the Arabidopsis thaliana and single cells development of the Medicago sativa. We have found that ROS is an important regulator of plants development and act through auxin signaling in the regulation of the root architecture. Moreover, ROS is required for cell divisions activity and lateral root development.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Regulator of the cells polarity===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The methods we developed have been also applied to investigation of the mechanism of the cell polarity induction in the Arabidopsis thaliana. Our data demonstrate for the first time that BFA at low dose (1 to 3 μM) have strong impact on auxin distribution, root growth, root apical meristem architecture and stem cells maintenance independently from internalizations of the membrane proteins.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Staff|Pasternak]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Staff|Pasternak]]</div></td></tr>
</table>WikiSysophttps://icbge.org.ua/en/index.php?title=Taras_Pasternak&diff=229&oldid=prevWikiSysop at 08:18, 3 February 20132013-02-03T08:18:12Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:18, 3 February 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== Research skills ==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Cellular biology skills:===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* protoplast culture</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* suspension culture</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* cell cycle investigation</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* plant tissue culture and transformation mediated by Agrobacterium tumefaciens or by biolistic bombardment</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* RNA in situ hybridization</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* free-hand sectioning and paraffin sectioning</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* whole-mounting in situ immunolocalization</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* laser scanning microscopy</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* cell and tissue staining with fluorescent dyes</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Molecular biology skill:===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* plasmid construction</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* DNA/RNA/protein extraction</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Southern, Northern and Western blotting</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* PCR, RT-PCR, Semi-quantitative RT-PCR, Quantitative RTPCR and Tail-PCR</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Bioinformatics skills:===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Sequence analysis</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* High-throughput image analysis</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Staff|Pasternak]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Staff|Pasternak]]</div></td></tr>
</table>WikiSysop