Date of birth: March 27, 1959, Mukachevo, Ukraine
Language skills: Russian, Ukrainian, English, German
Present address: Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany
E-mail: email@example.com or firstname.lastname@example.org
Personal web site: www.pasternak.pp.ua
|1983-1986||Ph.D. study in the Institute of Botany, National Academy of Sciences of the Ukraine, Kiev, under supervision of Prof. Yu.Yu. Gleba. PhD thesis "Genetic transformation of higher plants by microinjection", Ph.D. degree obtained in April, 1987.|
|1976-1981||Graduate course in the Kharkov State University, Radiophysical Faculty, Department of Biophysics, Master of Science degree obtained, diploma work in the Institute of Low Temperature, Academy of Sciences of the Ukraine, Kharkiv.|
|2004-2011||Guest docent in the Institute of Biology II/Botany University of Freiburg. Project: «Crosstalk between auxin and ROS in the regulation of the Arabidopsis development» (2004-2011).|
|2003||Guest professor in the University of Antwerp. Project: «The role of ascorbate and glutathione in the regulation of plant growth and development» (2003).|
|1998-2002||Research fellow in Institute of Plant Biology, Hungarian Academy of Science, Szeged. Projects: «Fluorescent microscopy as a tools for studying the early events in cell division and somatic embryogenesis in alfalfa root and leaf protoplasts» (1998); «Role of oxidative stress-inducing agents in auxin-mediated and H2O2 -dependent activation of cell division in cultured plant cells» (1999-2000); «Physiological analysis of role of retinoblastoma gene in alfalfa and tobacco» (2001-2002); «Possible role of nitric oxide in cell cycle and embryogenesis regulation in cultured alfalfa cells» (2002).|
|1997||UNESCO fellowship Institute of Botany, Hamburg (1997). Project: «In vitro plant regeneration from mature cereal organs».|
|1983-...||Research fellow in Institute of Cell Biology and Genetic Engineering, Kiev. Projects: «Genetic transformation of higher plants by microinjection» (1983-1987), «Genetic transformation of cereals» (1988-1994); «Chemical mutagenesis in cereals by mutagenic treatment of zygotic embryo cells» (1988-1994); «Molecular genetic analysis of oxidative stress in plants» (1994-1997).|
|1981-1983||Junior Research Fellow, Institute of Low Temperature, Kharkiv (1981-1983).|
Cellular biology skills:
- protoplast culture
- suspension culture
- cell cycle investigation
- plant tissue culture and transformation mediated by Agrobacterium tumefaciens or by biolistic bombardment
- RNA in situ hybridization
- free-hand sectioning and paraffin sectioning
- whole-mounting in situ immunolocalization
- laser scanning microscopy
- cell and tissue staining with fluorescent dyes
Molecular biology skill:
- plasmid construction
- DNA/RNA/protein extraction
- Southern, Northern and Western blotting
- PCR, RT-PCR, Semi-quantitative RT-PCR, Quantitative RTPCR and Tail-PCR
- Sequence analysis
- High-throughput image analysis
Investigation of the interaction between environmental factors, plant architecture and hormonal system of Medicago sativa L. and Arabidopsis thaliana L.
Together with my co-workers we have developed several methods to trace gene expression and organ morphology at different levels. These methods include:
- isolation and cultivation of the isolated protoplasts from root and leaf with further possibilities of direct embryogenesis;
- whole mounting molecules immune-localization at whole seedlings, organs and cellular level with further 3D organ/cells reconstruction
- creating virtual 3D nucleus map of Arabidopsis roots;
All these protocols have been used as tools to further investigation of interaction of environmental factors, low molecular weight antioxidants, inhibitor of cell polarization Breferldin A with hormonal system of Arabidopsis thaliana and Medicago sativa.
Low molecular weight antioxidant
We investigated interaction between GSH level and hormonal system by using as examples Arabidopsis thaliana root system and Medicago sativa organs and single cells.
The resulting data demonstrate for the first time that GSH impacts on auxin mediated responses as well as auxin transporters localization, hence serving as an auxin-response modulator. In the Medicago sativa we have found that hGSH have a different role than GSH: GSH involvement in the regulation of cell division, while hGSH play a significant role in the cell differentiation.
Reactive oxygen species
Next, we investigated interaction of reactive oxygen species with auxin in the regulation of root development in the Arabidopsis thaliana and single cells development of the Medicago sativa. We have found that ROS is an important regulator of plants development and act through auxin signaling in the regulation of the root architecture. Moreover, ROS is required for cell divisions activity and lateral root development.
Regulator of the cells polarity
The methods we developed have been also applied to investigation of the mechanism of the cell polarity induction in the Arabidopsis thaliana. Our data demonstrate for the first time that BFA at low dose (1 to 3 μM) have strong impact on auxin distribution, root growth, root apical meristem architecture and stem cells maintenance independently from internalizations of the membrane proteins.