Study of Artemisia tilesii Ledeb. "hairy" roots / Ostapchuk А., Matvieieva N. 2015

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Ostapchuk А., Matvieieva N.

In vitro A.tilesii plants (a); “hairy” root culture (b); HPLC study of artemisinin content (c); DPPH analysis of extracts from root lines (d)

We used Artemisia tilesii Ledeb. “hairy” root culture established after genetic transformation with Agrobacterium rhizogenes A4 wild strain and A. rhizogenes carried vector pCB124 with nptII and ifn-α2b genes. DPPH activity and artemisinin accumulation in “hairy” root lines were studied. HPLC analysis was performed to detect artemisinin content in transgenic root culture and in in vitro cultivated plants. The results obtained testify that artemisinin accumulated in amount up to 8 mg/g lyophilised A. tilesii “hairy” roots. Artemisinin content in the roots of aseptically cultivated plants was found to be 9 mg/g lyophilised plant material. DPPH activity of aqueous extracts of transgenic roots in some lines was higher then the same activity of extracts from the roots of the control plants. In consideration of the data obtained, Artemisia tilesii “hairy” roots are a promising plant material for biologically active substances production.

Keywords: Artemisia tilesii Ledeb., “hairy” root culture, artemisinin, DPPH

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