Iryna M. Gerasymenko

PhD

Scientific Associate of Department of genetic engineering.

Iryna Mykhajlivna GERASYMENKO

CURRICULUM VITAE

Institute of Cell Biology & Genetic Engineering

Natl. Academy of Sci. of Ukraine

Zabolotnogo str., 148, Kiev, 03143, Ukraine;

Tel/fax: +380 (44) 526 7104; 522 1787

E-mail: i-gerasimenko@ukr.net

http://icbge.org.ua/eng/Iryna_M._Gerasymenko

Year and Place of Birth: 1973, Kiev, Ukraine

Languages: Russian, Ukrainian - native, English – substantial, German – B2 (128 onDaF points)

Education, Scientific Degrees and Titles:
• Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, M. Sc. in Cell Biology and Genetic Engineering, 1996;
• PhD thesis "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures". Degree of Doktor der Naturwissenschaften obtained at the Biological Faculty of Johannes Gutenberg University of Mainz, Germany, February 8, 2002. Certified in Ukraine, January 15, 2003 (speciality molecular biology).

Professional Experience:
• 1996 – 1997: PhD student at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);
• 1997 –2002: research assistant at the Institute of Pharmacy (Mainz, Germany); 1997-1998: participation in the work on the project „Molecular Characterization of New Hybrid Plants and Cell Cultures“ supported by BMBF, (Ref. No. 0311269); 01.10.1998-31.03.1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”
• 2002 – up to date: research scientist (scientific co-worker) at the Institute of Cell Biology and Genetic Engineering (Kiev, Ukraine);
• 05-08.2006; 10-12.2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291) at the Institute of Pharmacy (Mainz, Germany).

Grant support:
• 1998 - 1999: DAAD stipendium for the project “Phytochemische Charakterisierung der transgenischen Wurzelkulturen einiger Apocynaceae Pflanzen”
• 2004 – 2006: INTAS Postdoctoral Young Scientist Fellowship “Characterization of enzymes of 19(S),20(R)-dihydroperaksine type alkaloid biosynthesis in Rauvolfia serpentina transgenic root culture” (INTAS Ref. Nr. 03-55-2291)
• 2007 - 2008 NAS of Ukraine Competition of Young Scientist Projects “Development of the protocol for expression and one stage purification of antituberculous antigens from hairy roots of Nicotiana species” (№0107U007145)
• 2012 - 2014 Dupont Young Professor Fellowship for the project “Effectiveness of plastome engineering to produce proteins and/or agriculture plants”

Teaching Experience: • Taras Shevchenko University, Kiev, Biological Faculty, Dept. of Cell Biology and Genetic Engineering, Dept. of General and Molecular Genetics, invited lecturer with courses “Plant genetics”, “Molecular phytopathology”

Computer Experience: Windows, MS Office, Adobe Photoshop, Corel Draw, Vector NTI, Internet Molecular Biology Database Servers – advanced user.

Awards: • Stipendium of National Academy of Science of Ukraine (2002)
• Stipendium of President of Ukraine (2008)

Fields of scientific interests: Plant Cell Culture / Plant biotechnology / Genetic transformation / Medicinal plants / Secondary metabolism regulation in plant cell and tissue cultures / Natural products: isolation, purification, structure elucidation / Secondary metabolism biosynthetic pathways, enzymes and genes / Pharmaceutical recombinant proteins / Expression of recombinant proteins in plant systems/ Plant Molecular Biology

Scientific Method Experience: complex of molecular biology protocols including DNA/RNA isolation and cloning, PCR- and RT-PCR techniques, protein extraction and purification, enzymatic activity assays, transient expression and genetic transformation of plants, in vitro plant cell culture, HPLC, FPLC.

Publications: 115 scientific publications including 32 publications in international peer review scientific journals, 2 patents of Ukraine;

H-index: 12 (Google Scholar, i10=12); 9 (Scopus, self-citation excluded)

Google Scholar Citation

Selected Scientific Publications:
1. Sheludko Yu., Gerasimenko I., Unger M., Kostenyuk I., and Stöckigt J. (1999) Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18, 911-918.
2. Warzecha H, Gerasimenko I, Kutchan TM, and Stöckigt J (2000) Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry, 54, 657-666.
3. Gerasimenko I., Sheludko Y., and Stöckigt J. (2001) 3-Oxo-rhazinilam: A new indole alkaloid from Rauvolfia serpentina x Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64, 114-116.
4. Stöckigt J., Sheludko Y., Unger M., Gerasimenko I., Warzecha H., Stöckigt D. (2002) High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionization mass spectrometric analysis of selected alkaloid groups. J. Chrom. A, 967, 85-113.
5. Gerasimenko I., Sheludko Y., Ma X., Stöckigt J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269, 2204-2213.
6. Sheludko Y., Gerasimenko I., Kolshorn H., Stöckigt J. (2002) New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture. J. Nat. Prod., 65, 1006-1010.
7. Gerasimenko I., Ma X., Sheludko Y., Mentele R., Lottspreich F., Stöckigt J. (2004) Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis. Bioorg. Med. Chem. 12, 2781-2786.
8. Sheludko Y. V., Sindarovska Y. R., Gerasymenko I. M., Bannikova M. A., Kuchuk N. V. (2007) Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotech. Bioeng. 96, 608-614.
9. Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal. Bioanal. Chem. 397, 2289-2293.
10. Luchakivskaya Y, Kishchenko O, Gerasymenko I, Olevinskaya Z, Simonenko Y, Spivak M, Kuchuk M. (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Rep. 30:407-415.
11. Sheludko Y.V. , Gerasymenko I.M. (2013) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262.
12. Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 208-217.
13. Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. (2015) Multiplex pcr assay for detection of human somatotropin and interferon alpha2b genes in plant material Cytol. Genet., 49(3), 147-151 DOI: 10.3103/S0095452715030068).
14. Gerasymenko I.M., Sakhno L.O., Kyrpa T.М., Ostapchuk A.N., Khadjiev T.A., Goldenkova-Pavlova I.V., Sheludko Y.V. (2015) Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl–lipid desaturases and thermostable lichenase. Russian J. Plant Physiol. 62(3), 283-291.
15. Gerasymenko, I.M., Sheludko, Y.V., Klebanovych, A.A., Rudas, V.A., Shakhovsky, A.M., Klein, T.M., Kuchuk, N.V. (2017) Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts. Transgenic research. 26 (1), 65-75. DOI: 10.1007/s11248-016-9980-2.
16. Gerasymenko I.M., Kleschevnikov V.V., Kedlian V.R., Sakhno L.O., Arbuzova I.A., Sheludko Y.V., Dosenko V.E., Kuchuk N.V. (2017) Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA. Cytol. Genet. 51(1), 1-7. doi:10.3103/s0095452717010054.
17. Gerasymenko, I.M., Sheludko Y.V. (2017) Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures. Biotech. Lett. 39, 1059-1067. DOI:10.1007/s10529-017-2336-z.