Laboratory of Natural Product Biosynthesis Systems

Laboratory was established in 2009 году

Laboratory Presentation

Group of Dr. Yuriy Sheludko performs researches in area of plant natural product biosynthesis. It includes studies on production of recombinant proteins in plants using transient and constitutive foreign gene expression. The aim of studies is understanding of transgene expression mechanisms in different types of plant systems (including undifferentiated cell cultures), selection of transgenic plants with agriculture valuable characteristics and development of biotechnological protocols for production of pharmaceutical proteins or plant based vaccines. The modern methods of molecular and cell biology and recombinant DNA protocols are applied for expression, vector construction and foreign DNA introduction into plant cell. Additionally, investigation of secondary metabolism in plants or in vitro cell systems of pharmacologically valuable species (Rauvolfia serpentina etc.), isolation, purification and identification of single compounds and enzymes is a focus of researches. Group studies influence of elicitors and stress factors on accumulation of secondary metabolites. In the course of researches a wide variety of analytical techniques are applied including HPLC, FPLC, flash chromatography, analytical and preparative thin layer chromatography and spectroscopy methods.

Structure:

Head of laboratory, PhD,Yuriy V. Sheludko
Scientific associate, PhD, Iryna M. Gerasymenko
Scientific associate, PhD, Yana R. Sindarovska
Principal engineer Mariya G. Mazur
PhD student, 1st rank engineer(1/2 rate) Tatiana M. Kyrpa
Diploma student, 1st rank engineer(1/2 rate) Iryna A. Arbuzova

News:

Oral presentation of Klebanovych A.A. "TRANSPLASTOMIC TOBACCO LINES CONTAINING uidA REPORTER GENE UNDER CONTROL OF DIFFERENT PROMOTER SEQUENCES", with co-authors Rudas V.A., Gerasymenko I.M., Sheludko Y.V., Vasylenko M.Y., Kuchuk N.V. from Laboratory of Natural Product Biosynthesis Systems and Department of genetic engineering as well as Klein T.M. from DuPont Pioneer AgBiotech was awarded by 1 Place Diploma during X INTERNATIONAL SCIENTIFIC CONFERENCE FOR STUDENTS AND PHD STUDENTS <<YOUTH AND PROGRESS OF BIOLOGY>> (Lviv, April, 8–11, 2014), section GENETICS AND BIOTECHNOLOGY. Abstracts of congress.

In 2012 Springer released book Biotechnology for Medicinal Plants: Micropropagation and Improvement, including review article Biosynthetic Potential of Hairy Roots for Production of New Natural Products prepared by members of the Laboratory of Natural Product Biosynthesis Systems Dr. Yuriy V. Sheludko and Dr. Iryna M. Gerasymenko]].

In the review authors focus on new substances produced in hairy roots as a result of tumorous root formation by itself or in combination with different treatments and/or genetic engineering manipulations and display that metabolite profile of transgenic roots may differ from that of parental plant, but this feature is not necessarily a disadvantage. Examples of new secondary metabolites from hairy root cultures, not known in parental plants, and products of heterologous gene expression are described. Authors suggest that transgenic roots may be regarded as a promising origin of natural product biodiversity.
Request reprint: ysheludko@ukr.net.

Honours and Awards:

President of Ukraine Viktor Fedorovych Yanukovych awarded member of the Laboratory of Natural Product Biosynthesis Systems Dr. Yana R. Sindarovska

20.04.2012 President of Ukraine Viktor Fedorovych Yanukovych awarded diploma of Annual President Prize for Young Scientists 2011 to member of the Laboratory of Natural Product Biosynthesis Systems Dr. Yana R. Sindarovska and to member of Department of genetic engineering Maxim Y. Vasylenko together with collegues from D.K.Zabolotnogo Institute of Microbiology and Virology O. Lozova and A. Demchenko for their collective scientific work "Obtaining of pharmaceutically valuable proteins in plants and studying on transgenic plant resistant to phytopathogenic viruses”. Pictures of the ceremony are here.

Iryna M. Gerasymenko was Awarded with DuPont Young Professor grant 2012 for research topic "Effectiveness of plastome engineering to produce proteins and/or agriculture plants".

Recent Publications:

• Sindarovska Y.R., Golovach I.S., Belokurova V.B., Gerasymenko I.M., Sheludko Y.V., Kuchuk N.V. (2014) Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression. Cytol. Genet. 48(4), 9-18.Request/Запросить копию у автора: ysheludko@ukr.net.
• Sheludko Y.V. , Gerasymenko I.M. (2012) Biosynthetic potential of hairy roots for production of new natural products. In: "Biotechnology for Medicinal Plants: Micropropagation and Improvement" Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer, Berlin, Heidelberg, 241-262. DOI: 10.1007/978-3-642-29974-2_10. Request/Запросить копию у автора: ysheludko@ukr.net.
• Sindarovska Y. R., Gerasymenko I. M., Kuchuk M. V., Sheludko Y. V. (2012) Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior. Biopolym. Cell., 28(№5), 368-373.fulltext Request/Запросить копию у автора: ysheludko@ukr.net.
• Сахно Л.А., Герасименко И.М., Комарницкий И.К., Шелудько Ю.В., Голденкова-Павлова И.В. (2012) Создание устойчивых к глифосату растений Brassica napus L., экспрессирующих десатуразу DesC цианобактерии Synechococcus vulcanus. Biopolym. Cell., 28(6), 449-455. abstract Request/Запросить копию у автора: ysheludko@ukr.net.
• Вячеславова А.О., Мустафаев О.Н., Тюрин А.А., Шимшилашвили Х.Р., Бердичевец И.Н., Шаяхметова Д.М., Голденков М.А., Фадеев В.С., Шелудько Ю.В., Голденкова-Павлова И. В. (2012) Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях. Генетика, 48(№9), 1046-1056. abstract. Request/Запросить копию у автора: ysheludko@ukr.net.
• Gerasymenko I.M., Sakhno L.O., Mazur M.G., Sheludko Y.V. (2012) Multiplex PCR assay for detection of human interferon alpha2b gene in transgenic plants. Cytology and Genetics 46 (N4), 197-201.DOI: 10.3103/S009545271204007X Request/Запросить копию у автора: ysheludko@ukr.net.
• Yu. Luchakivskaya, O. Kishchenko, I. Gerasymenko, Z. Olevinskaya, Yu. Simonenko, M. Spivak and M. Kuchuk (2011) High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants. Plant Cell Reports DOI: 10.1007/s00299-010-0942-5
• Ovcharenko, O., V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) O.E. Schulz by protoplast fusion." Plant Cell, Tissue and Organ Culture: 105, 21-27 doi.org/10.1007/s11240-010-9833-2
• Sindarovska Y.R., Gerasymenko I.M, Sheludko Y.V., Olevinskaja Z.M., Spivak N.Y., Kuchuk N.V. (2010) Production of human interferon alpha 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression. Cytol Genet 44 (N5), 313-316
• Berdichevets, I., H. Shimshilashvili, I. Gerasymenko, Y. Sindarovska, Y. Sheludko and I. Goldenkova-Pavlova (2010) Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Analytical and Bioanalytical Chemistry 397, 2289-2293DOI 10.1007/s00216-010-3770-0.
• Lystvan, K., V. Belokurova, Y. Sheludko, J. Ingham, V. Prykhodko, O. Kishchenko, E. Paton and M. Kuchuk (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell, Tissue and Organ Culture (PCTOC) 101, 99-103.
• Sheludko Y. V., (2010) Recent advances in plant biotechnology and genetic engineering for obtaining of secondary metabolites. Cytol Genet 44 (N1), 52-60.
• Герасименко И. М., Головач И. С., Кищенко Е. М., Сахно Л. А., Синдаровская Я. Р., Шимшилашвили Х. Р., Шелудько Ю.В., Голденкова-Павлова И.В. (2010) Получение и анализ трансгенных растений, несущих гены 9 и 12 десатураз цианобактерий. Информационный вестник ВОГиС 14 (1), 127-133.